Overview:
14-3-3θ(alsoknownastyrosine3-monooxygenase/tryptophan5-monooxygenaseactivationprotein,thetapolypeptide)isamemberofthe14-3-3familyofproteinswhichmediatesignaltransductionbybindingtophosphoserine-containingproteins.ThroughinteractionwithASK1,c-junNH-terminalkinase,andp38mitogen-activatedproteinkinase(MAPK),14-3-3θplaysanimportantroleincontrollingapotopsis(1).Inducedexpressionof14-3-3θproteinhasbeenreportedinpatientswithamyotrophiclateralsclerosis.Additionally,14-3-3θhasbeenobservedtomediatenucleocytoplasmicshuttlingoftheNprotein(coronavirusnucleocapsidprotein)whichcausessevereacuterespiratorysyndrome(2).
GeneAliases:
YWHAQ,1C5,HS1,14-3-3
GenbankNumber:
NM_006826
References:
1.Lau,J.M.etal:The14-3-3tauphosphoserine-bindingproteinisrequiredforcardiomyocytesurvival.MolCellBiol.2007,27(4):1455-66.2.Thesevereacuterespiratorysyndromecoronavirusnucleocapsidproteinisphosphorylatedandlocalizesinthecytoplasmby14-3-3-mediatedtranslocation.JVirol.2005Sep;79(17):11476-86.
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本人目前需从电鳗放电器官中提取乙酰胆碱受体蛋白,提纯需要的步骤和电鳗器官都可以提供,有没有提供外包提纯服务的公司或者实验室。
算上其他生物24种
大概是记不太清了
NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."

