Overview:
14-3-3zeta(alsoknownastyrosine3-monooxygenase/tryptophan5-monooxygenaseactivationprotein,zetapolypeptide)isamemberofthe14-3-3familyofproteinswhichmediatesignaltransductionbybindingtophosphoserine-containingproteins.14-3-3zetaproteinplaysakeyroleincancerBIOLOGybybeinganimportantregulatorofmajorcellularprocessessuchasproliferation,differentiation,senescenceandapoptosis(1).14-3-3zetaproteinhasbeenshowntointeractwiththeIRS1protein,suggestingaroleforthisproteininregulatinginsulinsensitivitybyinterruptingtheassociationbetweentheinsulinreceptorandIRS1(2).
GeneAliases:
14-3-3zeta,YWHAZ,KCIP-1,MGC111427,MGC126532,MGC138156
GenbankNumber:
NM_003406
References:
1.Li,Z.etal:Down-regulationof14-3-3zetasuppressesanchorage-independentgrowthoflungcancercellsthroughanoikisactivation.ProcNatlAcadSciUSA.2008;105(1):162-7.2.Niemantsverdriet,M.etal:Cellularfunctionsof14-3-3zetainapoptosisandcelladhesionemphasizeitsoncogeniccharacter.Oncogene.2008;27(9):1315-9.2007.
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本人目前需从电鳗放电器官中提取乙酰胆碱受体蛋白,提纯需要的步骤和电鳗器官都可以提供,有没有提供外包提纯服务的公司或者实验室。
算上其他生物24种
大概是记不太清了
NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."

