
SMARTerThruPLEXPlasma-SeqkitsarepoweredbySMARTerThruPLEXchemistrytogeneratehigh-performanceNGSlibrariesfromcell-freeDNAisolatedfromplasma.Thechemistryhasbeenspecificallyoptimizedforcell-freeDNAtomaximizelibrarycomplexityandtopreservetheGCrepresentationoftheinputsample.LibrariescanbeusedforCNVanalysis,whole-genomesequencingapplications,orenrichmentusingyourcustompanelsorleADIngtargetenrichmentplatforms,suchasAgilentSureSelectandRocheNimbleGenSeqCapEZ.
TheSMARTerThruPLEXPlasma-SeqKithasbeenreconfiguredfromtheoriginalversionsofThruPLEXkitsanddoesnotincludeindexes.CompatIBLeSMARTerIndexkitsaredescribedbelowandcanbepurchasedhere.
SMARTerThruPLEXPlasma-SeqkitsarepoweredbySMARTerThruPLEXchemistrytogeneratehigh-performanceNGSlibrariesfromcell-freeDNAisolatedfromplasma.Thechemistryhasbeenspecificallyoptimizedforcell-freeDNAtomaximizelibrarycomplexityandtopreservetheGCrepresentationoftheinputsample.LibrariescanbeusedforCNVanalysis,wholegenomesequencingapplications,orenrichmentusingyourcustompanelsorleadingtargetenrichmentplatforms,suchasAgilentSureSelectandRocheNimbleGenSeqCapEZ.
TheSMARTerThruPLEXPlasma-SeqKithasbeenreconfiguredfromtheoriginalversionsofThruPLEXkitsanddoesnotincludeindexes.CompatibleSMARTerIndexkitsaredescribedbelowandcanbepurchasedhere.
LibrariespreparedwithSMARTerThruPLEXPlasma-Seqkitsprovideaddedsensitivitytobetterunderstandgeneticvariationsfoundwithintheplasmasamples.Plasma,alongwithotherliquidbiopsies,hasgainedsignificantinterestintheresearchcommunityasavaluableandeasilyavailablesampletype.Thesecommunitiesincludetranslationalresearchinoncologyanddevelopmentalresearchinvolvingthefetalfractionderivedfrommaternalplasma.
SMARTerDNAHTDualIndexkits
SMARTerDNAHTDualIndexkitsaredesignedforusewithSMARTerThruPLEXandSMARTerPicoPLEXlibrarypreparationkitstoconstructlibrariesformultiplexedsequencingonIlluminasequencers.ThesekitscontainindexedPCRprimerscarryingtheIlluminaNextera®XTv2indexsequencesandofferatotalof384dualindexesformultiplexingofupto384samples.TheindexedPCRprimersaresuppliedpredispensedinfourdifferentbarcodedindexplates,eachofwhichcontainsone-fourthofthepossibledualindexcombinations(Cat.#R400660–R400663);orinasetof24individualtubes,eachcontainingadifferentdualindexcombination(Cat.#R400664).Eachdualindextube(24N)containssufficientvolumeforuptotwouses.Eachwellofadualindexplate(96NSetsA–D)containssufficientvolumeforasingleuse.
SMARTerDNAUniqueDualIndexkits
SMARTerDNAUniqueDualIndexkitsaredesignedforusewithSMARTerThruPLEXandSMARTerPicoPLEXlibrarypreparationkitstoconstructlibrariesformultiplexedsequencingonIlluminasequencers.ThesekitscontainindexedPCRprimerscarryingthe"IDTforIlluminaUD"indexsequencesandofferatotalof96dualindexesformultiplexingofupto96samples.TheindexedPCRprimersaresuppliedpre-dispensedinfourdifferentsetsof24individualtubes,eachofwhichcontainsone-fourthofthepossibledualindexcombinations(Cat.Nos.R400665–R400668).Eachdualindextube(24USetsA–D)containssufficientvolumeforuptotwouses.
SMARTerDNASingleIndexkits
SMARTerDNASingleIndexkitsaredesignedforusewithSMARTerThruPLEXandSMARTerPicoPLEXlibrarypreparationkitstoconstructlibrariesformultiplexedsequencingonIlluminasequencers.ThesekitscontainindexedPCRprimerscarryingthe"TruSeqLTsetA"indexsequencesandofferatotalof12singleindexesformultiplexingofupto12samples.TheindexedPCRprimersaresuppliedpre-dispensedinfourdifferentsetsof12individualtubes,eachcontainingadifferentindexsequence(Cat.Nos.R400695andR400697).Eachsingleindextube(12SSetsA–B)containssufficientvolumeforuptoeightuses.
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算上其他生物24种
大概是记不太清了

