
Theproductslistedonthispagearebeingphasedout.Replacementproductsarealreadyavailable:
WerecommendreplacingTaqfullDNApolymerasewiththenewerTaKaRaTaqDNApolymerase,whichisarecombinantversionoftheThermostable,full-lengthTaq.TakaraTaqisavailableasindividualcomponents,inahot-startformulation,orasacompletekit.
Theproductslistedonthispagearebeingphasedout.Replacementproductsarealreadyavailable:WerecommendreplacingTaqfullDNApolymerasewiththenewerTaKaRaTaqDNApolymerase,whichisarecombinantversionofthethermostable,full-lengthTaq.TakaraTaqpolymeraseisavailableasindividualcomponents,inahot-startformulation,orasacompletekit.
TaqfullDNApolymeraseisanultra-pure,highlyefficient,full-length,recombinantversionoftheThermusaquaticus(Taq)YT1DNAPolymerase(94kD)(Engelkeetal.1990).Thisenzymepossessestwoimportantcatalyticactivities:a5"→3"polymeraseactivity,andadouble-strand-specific5"→3"exonucleaseactivity.Likeotherfull-lengthTaqDNAPolymerases,thisenzymelacks3"→5"exonuclease(proofreADIng)activity.ThisenzymeissuitableforanygeneralPCRamplificationprocedure.ItamplifiesevenraretargetsfrombacterialandplasmidDNA,CDNA,andcomplexgenomicDNA.
TaqfullDNApolymeraseisavailableinseveralformats:
- Individualcomponentsofpolymeraseenzymemix(Cat.#639233and639234)—separatetubesofTaqfullDNApolymeraseand10Xbuffer
- TaqfullDNApolymeraseinacompletekit(Cat.#639235)—includesTaq,10Xbuffer,MgCl2,dNTPs,controlgenomicDNAtemplate,andprimermix
- Ahot-startpolymeraseformulationinacompletekit(Cat.#639231)—includeshot-startTaq,10Xbuffer,MgCl2,dNTPs,controlgenomicDNAtemplate,andprimermix
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NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."
算上其他生物24种
大概是记不太清了
本人目前需从电鳗放电器官中提取乙酰胆碱受体蛋白,提纯需要的步骤和电鳗器官都可以提供,有没有提供外包提纯服务的公司或者实验室。

