
Acetyl Lysine Antibody, Agarose -5mg
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Product Description | A high density of the acetylated lysine antibody is immobilized to agarose beads. The product can be utilized as an affinity matrix for rapid isolation and purification of proteins/peptides with acetyl lysine residues. | ![]() The acetylated protein signal as determinated by ELISA titration (OD50 titer). The acetlated proteins were eluted from 1 mL the Rabbit anti-Acetyllysine Agarose (ICP0388) and immobilized on microrplate, followed by detection with Mouse anti-Acetylated lysine HRP. Rabbitt anti-Biotin Agarose (1 mL) was utilized as the negative control. ![]() The maximum binding of acetylated BSA with ICP0388-5MG. -50 µl of ICP0388-5MG were incubated with 1 mg of acetylate BSA in a 1 ml tube for 60 min. - After washing with PBST 4 times, the bound acetylated BSA was eluted with 1 ml of 0.5 M HCl -5 µl / lane of the eluted acetylated BSA was resolved by SDS-PAGE and blotted wih monoclonal Mouse anti-Acetylated Lysine (ICP0390) -ECL exposure 3 seconds | |
Formulation | 0.5 mL of agarose beads suspended in 1 mL of slurry | ||
Antibody Immobilized | 10 mg/mL antibody is covalently linked through amide bonds with NHS activated-SMCC, which further conjugates to thiolated agarose beads via thiol ether bonds. | ||
Specificity | This antibody affinity matrix selectively captures proteins/peptides with acetylated lysine residues (N-epsilon). No cross creation to methylated proteins/peptides. | ||
BindingCapacity | Approximately 0.5-1 mg of acetylated histone / mL slurry. | ||
Applications | Rapid isolation and purification of proteins/peptides with acetylated lysine residues from cell lysate or protease-digested mixtures. | ||
Scientific Description | Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of acetylated proteins/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the acetylated species for further MS/MS identification. | ||
Storage & Stability | Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Do not aliquot and shake thoroughly before use.Expiration date is one year from date of shipping if properly stored. | ||
Product Specific References |
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算上其他生物24种
大概是记不太清了
NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."