Description

Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.

Cultrex®RGFBMEisaBasementMembraneMatrix thathasbeendeveloped,producedandqualifiedforgeneralcellcultureapplications. Cultrex®RGFBMEisprocessedtoreducematrix-associatedgrowthfactorsandprovideamoredefinedmodelsystem.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof12–18mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
• GelstABIlitytestedforaperiodof14daysat37°C.
• BIOLOGicalactivitydeterminedforeachlot:
  • Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.

GPERMediatesActivationofHIF1{alpha}/VEGFSignalingbyEstrogens
ErnestinaMariannaDeFrancesco,MichelePellegrino,MariaFrancescaSantolla,RosamariaLappano,EmiliaRicchio,SergioAbonante,andMarcelloMaggiolini
CancerRes.,Aug2014;74:4053–4064
http://cancerres.aacrjournals.org/cgi/content/abstract/74/15/4053

Vesicle-associatedMembraneProtein2(VAMP2)butNotVAMP3MediatescAMP-stimulatedTraffickingoftheRenalNa+-K+-2Cl-Co-transporterNKCC2inThickAscendingLimbs
PauloS.Caceres,MarielaMendez,andPabloA.Ortiz
J.Biol.Chem.,Aug2014;289:23951–23962.
http://www.jbc.org/cgi/content/abstract/289/34/23951

TheM33GProtein-CoupledReceptorEncodedbyMurineCytomegalovirusIsDispensableforHematogenousDisseminationbutIsRequiredforGrowthwithintheSalivaryGland
FabiolaM.Bittencourt,Shu-EnWu,JamesP.Bridges,andWilliamE.Miller
J.Virol.,Oct2014;88:11811–11824.
http://jvi.asm.org/cgi/content/abstract/88/20/11811

ImmunoevasivePericytesFromHumanPluripotentStemCellsPreferentiallyModulateInductionofAllogeneicRegulatoryTCells
HagitDomev,IrinaMilkov,JosephItskovitz-Eldor,andAyeletDar
StemCellsTransMed,Oct2014;3:1169–1181.
http://stemcellstm.alphamedpress.org/cgi/content/abstract/3/10/1169

PTENinhibitsPREX2-catalyzedactivationofRAC1torestraintumorcellinvasion
SarahM.Mense,DouglasBarrows,CindyHodakoski,NicoleSteinbach,DavidSchoenfeld,WilliamSu,BenjaminD.Hopkins,TaoSu,BarryFine,HaninaHibshoosh,andRamonParsons
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“Effectsofulipristalacetateonhumanembryoattachment
andendometrialcellgeneexpressioninaninvitro
co-culturesystem”
“C.Berger,N.R.Boggavarapu,J.Menezes,P.G.L.Lalitkumar,andK.Gemzell-Danielsson”
Hum.Reprod.,Apr2015;30:800–811.
http://humrep.oxfordjournals.org/cgi/content/abstract/30/4/800

“TheHippoPathwayEffectorYAPRegulatesMotility,
Invasion,andCastration-ResistantGrowthofProstate
CancerCells”
“LinZhang,ShupingYang,XingchengChen,SethStauffer,FangYu,SubodhM.Lele,
KaiFu,KaustubhDatta,NicholasPalermo,YuanhongChen,andJixinDong”
Mol.Cell.Biol.,Apr2015;35:1350–1362.
http://mcb.asm.org/cgi/content/abstract/35/8/1350

ConditionedMediumFromHumanAmnioticMesenchymalStromalCellsLimitsInfarctSizeandEnhancesAngiogenesis
“PatriziaDanieli,GiuseppeMalpasso,MariaChiaraCiuffreda,ELISAbettaCervio,LauraCalvillo,FrancescoCopes,FedericaPisano,ManuelaMura,LennaertKleijn,
RudolfA.deBoer,GianlucaViarengo,VittorioRosti,ArsenioSpinillo,MariannaRoccio,andMassimilianoGnecchi”
StemCellsTransMed,May2015;4:448–458.
http://stemcellstm.alphamedpress.org/cgi/content/abstract/4/5/448

LossofPPARinendothelialcellsleadstoimpairedangiogenesis
SannaVattulainen-Collanus,OyediranAkinrinade,MolongLi,MinnaKoskenvuo,CaiyunGraceLi,ShailajaP.Rao,ViniciodeJesusPerez,KeYuan,HirofumiSawada,JuhaW.Koskenvuo,CristinaAlvira,MarleneRabinovitch,andTero-PekkaAlastalo
J.CellSci.,Feb2016;129:693–705.
http://jcs.biologists.org/cgi/content/abstract/129/4/693

“FluorescentImage–GuidedSurgerywithanAnti-ProstateStemCell
Antigen(PSCA)DiabodyEnablesTargetedResectionofMouseProstateCancerXenograftsinRealTime”
GeoffreyA.Sonn,AndrewS.Behesnilian,ZiyueKarenJiang,KirstinA.Zettlitz,EricJ.Lepin,LaurentA.Bentolila,ScottM.Knowles,DanielLawrence,AnnaM.Wu,andRobertE.Reiter
Clin.CancerRes.,Mar2016;22:1403–1412.
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/6/1403

“Grainyhead-like2downstreamtargetsacttosuppressepithelialto-
mesenchymaltransitionduringneuraltubeclosure”
HeatherJ.RayandLeeA.Niswander
Development,Apr2016;143:1192–1204.
http://dev.biologists.org/cgi/content/abstract/143/7/1192

“SrcasaTherapeuticTargetinBiliaryTractCancer”
“Ah-RongNam,Ji-WonKim,JiEunPark,Ju-HeeBang,MeiHuaJin,Kyung-HunLee,Tae-YongKim,
Sae-WonHan,Seock-AhIm,Tae-YouKim,Do-YounOh,andYung-JueBang”
Mol.CancerTher.,Jul2016;15:1515–1524.
http://mct.aacrjournals.org/cgi/content/abstract/15/7/1515

Vesicle-associatedMembraneProtein3(VAMP3)MediatesConstitutiveTraffickingoftheRenalCo-transporterNKCC2inThickAscendingLimbs:ROLEINRENALFUNCTIONANDBLOODPRESSURE
PauloS.Caceres,MarielaMendez,MohammedZ.Haque,andPabloA.Ortiz
J.Biol.Chem.,Oct2016;291:22063–22073
http://www.jbc.org/cgi/content/abstract/291/42/22063

TransposonmutagenesisidentifiesgenesthatcooperatewithmutantPteninbreastcancerprogression
“RobertoRangel,Song-ChoonLee,KennethHon-KimBan,LilianaGuzman-Rojas,MichaelB.Mann,Justin
Y.Newberg,TakahiroKodama,LeslieA.McNoe,LuxmananSelvanesan,JerroldM.Ward,AlistairG.Rust,
Kuan-YewChin,MichaelA.Black,NancyA.Jenkins,andNealG.Copeland”
PNAS,Nov2016;113:E7749–E7758.
http://www.pnas.org/cgi/content/abstract/113/48/E7749

“PreclinicalEfficacyoftheAuristatin-BasedAntibody–DrugConjugate
BAY1187982fortheTreatmentofFGFR2-PositiveSolidTumors”
“AnetteSommer,CharlotteKopitz,ChristophA.Schatz,CarlF.Nising,ChristophMahlert,Hans-Georg
Lerchen,BeatrixStelte-Ludwig,StefanieHammer,SimoneGreven,JoachimSchuhmacher,Manuela
Braun,RuprechtZierz,SabineWittemer-Rump,AxelHarrenga,FrankDittmer,FrankReetz,Heiner
Apeler,RolfJautelat,HungHuynh,KarlZiegelbauer,andBertoltKreft”
CancerRes.,Nov2016;76:6331–6339
http://cancerres.aacrjournals.org/cgi/content/abstract/76/21/6331

“WntSignalingPromotesBreastCancerbyBlockingITCH-Mediated
DegradationofYAP/TAZTranscriptionalCoactivatorWBP2”
“ShenKiatLim,SsuYiLu,Shin-AeKang,HockJinTan,ZilinLi,ZhenNingAdrianWee,JyeSweiGuan,
VishnuPriyankaReddyChichili,J.Sivaraman,ThomasPutti,AyeAyeThike,PuayHoonTan,Marius
Sudol,DavidM.Virshup,SiewWeeChan,WanjinHong,andYoonPinLim”
CancerRes.,Nov2016;76:6278–6289.
http://cancerres.aacrjournals.org/cgi/content/abstract/76/21/6278

Theinterplaybetweenhypoxia,endothelialandmelanomacellsregulatesvascularizationandcellmotilitythroughendothelin-1andvascularendothelialgrowthfactor
Spinella,F.,Caprara,V.,Cianfrocca,R.,Rosano,L.,DiCastro,V.,Garrafa,E.,Natali,P.G.,andBagnato,A.Theinterplaybetweenhypoxia,endothelialandmelanomacellsregulatesvascularizationandcellmotilitythroughendothelin-1andvascularendothelialgrowthfactor41687
Carcinogenesis,Feb2014;10.1093/carcin/bgu018.
http://carcin.oxfordjournals.org/cgi/content/abstract/bgu018v2

HarvestofSuperficialLayersofFatWithaMicrocannulaandIsolationofADIposeTissue-DerivedStromalandVascularCells
Trivisonno,A.,DiRocco,G.,CanNISTra,C.,Finocchi,V.,TorresFarr,S.,Monti,M.andToietta,G.HarvestofSuperficialLayersofFatWithaMicrocannulaandIsolationofAdiposeTissue-DerivedStromalandVascularCellsJournalArticle
AestheticSurgeryJournal,May2014;34:601–613.
http://aes.sagepub.com/cgi/content/abstract/34/4/601