[图文]常用制药设备ppt课件
货号: | hz158Hu01 |
供应商: | 上海沪震 |
数量: | 100 |
英文名: | RecombinantS100CalciumBindingProteinA14(S100A14) |
保质期: | 12个月 |
保存条件: | -20℃ |
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!
Organismspecies Homosapiens(Human)
ProductNo. RPD158Hu01
Source Prokaryoticexpression
Host E.coli
Purity >90%
UOM 50ug
PredictedMolecularMass 40.2kDa
Applications SDS-PAGE;WB;ELISA;IP.
EndotoxinLevel <1.0EUper1µg(determinedbytheLALmethod)
S100钙结合蛋白A14(S100A14)重组蛋白Residues Met1~Ala91withtwoN-terminalTags,His-tagandGST-tag
Formulation SuppliedaslyophilizedforminPBS,pH7.4,containing5%trehalose,0.01%sarcosyl.
SEQUENCES
ThetargetproteinisfusedwithtwoN-terminalTags,His-tagandGST-tag,itssequenceislistedbelow.
MGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKETLTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGEA
USAGE
ReconstituteinsterilePBS,pH7.2-pH7.4.
STORAGEANDSTABILITY
Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.
StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationS100钙结合蛋白A14(S100A14)重组蛋白andprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
AbouttheMarker(complimentary)
EffectiveSizeRange:10kDato70kDa.
Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.
温馨提示:不可用于临床治疗。
免责声明 本文仅代表作者个人观点,与本网无关。其创作性以及文中陈述文字和内容未经本站证实,对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺,请读者仅作参考,并请自行核实相关内容。
版权声明 未经蚂蚁淘授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的,应该授权范围内使用,并注明“来源:蚂蚁淘”。违反上述声明者,本网将追究其相关法律责任。
发布于 : 2018-03-20
阅读(204)
▍
相关分类
▍
相关文章