货号：	hz158Hu01
供应商：	上海沪震
数量：	100
英文名：	RecombinantS100CalciumBindingProteinA14(S100A14)
保质期：	12个月
保存条件：	-20℃

S100钙结合蛋白A14(S100A14)重组蛋白RecombinantS100CalciumBindingProteinA14(S100A14)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!
Organismspecies   Homosapiens(Human)
ProductNo.   RPD158Hu01
Source   Prokaryoticexpression
Host   E.coli
Purity   >90%
UOM   50ug
PredictedMolecularMass   40.2kDa
Applications   SDS-PAGE;WB;ELISA;IP.
EndotoxinLevel   <1.0EUper1µg(determinedbytheLALmethod)
S100钙结合蛋白A14(S100A14)重组蛋白Residues   Met1~Ala91withtwoN-terminalTags,His-tagandGST-tag
Formulation   SuppliedaslyophilizedforminPBS,pH7.4,containing5%trehalose,0.01%sarcosyl.
SEQUENCES
ThetargetproteinisfusedwithtwoN-terminalTags,His-tagandGST-tag,itssequenceislistedbelow.
MGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKETLTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGEA
USAGE
ReconstituteinsterilePBS,pH7.2-pH7.4.
STORAGEANDSTABILITY
Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.
StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationS100钙结合蛋白A14(S100A14)重组蛋白andprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
AbouttheMarker(complimentary)
EffectiveSizeRange:10kDato70kDa.
Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.