![ACROBiosystems/Influenza A [A/Shanghai/2/2013(H7N9)] Hemagglutinin (HA) Protein, His Tag/1mg/HA9](images/ACROBiosystems/HA9-V5227.jpg)
- SynonymHA,Hemagglutinin
- SourceInfluenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (HA9-V5227) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Gly 338, Leu 341 - Asp 523 (Accession # R4NN21-1).Predicted N-terminus: Asp 19Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 56.7 kDa. The protein migrates as 63-75 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
![Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) SDS-PAGE gel](images/ACROBiosystems/HA9-V5227.jpg)
Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
![Influenza A [A/Shanghai/2/2013(H7N9)] HA, His TagInfluenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) ELISA bioactivity](images/ACROBiosystems/HA9-V5227-E1.jpg)
Immobilized Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL (QC tested).
- Citations
Proinflammatory effects of the hemagglutinin protein of the avian influenza A (H7N9) virus and microRNA‑mediated homeostasis response in THP‑1 cells
Authors: Zhang S, et al.
Journal: Mol Med Rep 2015
Application: Cell Culture
Request for Full-text
- BackgroundInfluenza, commonly known as "the flu", is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. The serotypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are: H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, H10N7, H7N9. H7N9 is a serotype of the species Influenzavirus A (avian influenza virus or bird flu virus). H7 normally circulates amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in 2013 in China.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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①乙型肝炎表面抗原—抗体系统(HBsAg/抗—HBs);
②乙型肝炎核心抗原—抗体系统(HBcAg/抗—HBc):
③乙型肝炎e抗原—抗体系统(HBeAg);
④乙型肝炎Dane颗粒抗原—抗体系统
⑤乙型肝炎δ抗原—抗体系统(δ/抗—δ)。
临床意义 1.HBsAg:血清中检测到HBsAg ,表示体内感染了HBV,因而是一种特异性标志。HBsAg阳性见于:①急性乙型肝炎的潜伏期或急性期(大多短期阳性);②HBV致的慢性肝病、迁延性和慢性活动性肝炎、肝炎后肝硬化或原发性肝癌等。③无症状携带者
2.抗HBs:表示曾感染过HBV,不论临床上有无肝炎症状表现,均已得到恢复,并且对HBV有一定的免疫力。
3.HBcAg与抗HBc:由于 HBcAg主要存在于肝细胞核内,并仅存在于Dane颗粒中。因此,对病人血清不能检测HBcAg,而测抗HBc。血清内抗HBc阳性反映:①新近有过HBV感染;②体内有HBV增殖;③有助于诊断急性或慢性乙型肝炎,特别是少数病例就诊时已处于急性恢复期早期,HBsAg已从血中消失,此时血中仅有抗HBc存在,因此,对恢复期患者可作病因追索。
4.HBcAg和抗HBe:HBcAg的存在常表示病人血液有感染性。 HBcAg阳性揭示病人肝脏可能有慢性损害,对预后判断有一定帮助。抗HBe阳性对病人可能有一定的保护力。展开
1、存在于细胞外环境时,不显复制活性,但保持感染活性,是病毒体或病毒颗粒形式。
2、抗原,是指能够刺激机体产生(特异性)免疫应答,并能与免疫应答产物抗体和致敏淋巴细胞在体外结合,发生免疫效应(特异性反应)的物质
流感病毒的基因组包含________基因,其中__________蛋白可作为__________的结构基础.
我不知道第一空如何填,第二空是否应该填"HA蛋白"?第3空是否应该填"突变"?
多谢各位啊!
新年快乐!
万事如意!
核心抗原由183个或185个氨基酸组成,高度磷酸化,是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面,被表面抗原覆盖,故不易在血循环中检出。核心抗原具有强免疫原性,可诱导很强的体液免疫和细胞免疫,刺激机体产生抗-HBc。
e抗原为可溶性蛋白质,传染性强,游离存在于血液中,虽然很早就被发现,在病理上认为是HBV复制以具有强感染性的一个指标,但其功能尚不清楚。抗-HBe的出现,是预后良好的征象。向左转|向右转
患者,双手扶墙,脚尖着地;医生做好消毒,戴上手套,利用放血针(可以利用测血糖的放血针),在委中穴点刺放血,利用拔罐器把瘀血抽出来,直到见到新鲜血液为止。委中穴是解毒大穴。
中医强调治症不治病,虽然身体还有病毒,但是,只要症状能够解除,不影响正常生活和工作,就可以了。
需要强调:医生,一定要做好消毒、保护,防止病毒感染。 另外,可以把艾滋病作为一种疫病,尝试五苓散;五苓散是用来治疗疫病的。
所以说,感染同一种病毒,每个人识别的表位可能不一样。

