| Allergen: | Biotinylated nAra h 2 (Arachis hypogaea allergen 2) |
| Unit: | 1mg |
| Source: | Light roasted peanut flour (Runner cultivar) |
| Mol. Wt: | 17-19 kD doublet |
| Purification: | From delipidated peanut extract by multi-step chromatography. Purity on silver stained SDS-PAGE >95%. |
| Concentration: | See product insert. By Advanced Protein Assay (Cytoskeleton Inc., Denver, CO) |
| Formulation: | Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Sterile filtered. |
| Storage: | Store at -20ºC |
| Notes: | (1) Ara h 2 appears as a doublet (4). (2) Protect from exposure to light. (3) Avoid repeated freeze-thaw cycles. |
| PDB: | |
| Product Resources: | BiotinNatural Ara h 2 Certificate of Analysis |
| Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. | |
| References: | |
Burks AW, Williams LW, Connaughton C, Cockrell G, O’Brien TJ, Helm RM. Identification and characterization of a second major peanut allergen, Ara h II, with use of the sera of patients with atopic dermatitis and positive peanut challenge. J Allergy Clin Immunol 1992;90:962-9. | |
Sen M, Kopper R, Pons L, Abraham EC, Burks W, Bannon GA. Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-Binding epitopes. J Immunol 2002;169:882-7. | |
| Flinterman AE, van Hoofen E, den Hartog Jager CF, Koppelman S, Pasmans SG, Hoekstra MO, Bruijnzeel-Koomen CA, Knulst AC, Knol EF. Children with peanut allergy recognize predominantly Ara h2 and Ara h 6, which remains stable over time. Clin Exp Allergy 2007;37:1221-8.. | |
McDermott RA, Porterfield HS, El Mezayen R, Burks AW, Pons L, Schlichting DG, Solomon B, Redzic, JS, Harbeck RJ, Duncan MW, Hansen KC, Dreskin SC. Contribution of Ara h 2 to peanut-specific, immunoglobulin E-mediated, cell activation. Clin Exp Allergy 2007;37:752-763. | |
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本是小菜鸟,目前研究某个比较新的蛋白对内质网应激的影响,需要抑制这个蛋白的表达看下游信号的表达情况,但是无论如何夜找不到抑制剂,做RNA干扰的话经费就超出预算了!急求各位大神,帮忙出个解决方案吧!非常感谢!
作为一种 RNA 导向的 dsDNA 结合蛋白,Cas9 效应物核酸酶是已知的第一个统一因子(unifying factor),能够共定位 RNA、DNA 和蛋白,从而拥有巨大的改造潜力。将蛋白与无核酸酶的 Cas9( Cas9 nuclease-null)融合,并表达适当的 sgRNA ,可靶定任何 dsDNA 序列,而 sgRNA 的末端可连接到目标DNA,不影响 Cas9 的结合。因此,Cas9 能在任何 dsDNA 序列处带来任何融合蛋白及 RNA,这为生物体的研究和改造带来巨大潜力。向左转|向右转
http://www.addgene.org/crispr/guide/
请有经验的战友指点,若可以最好能提供几篇应用动物进行RNA干扰研究的文章.
非常感谢!
http://www.nature.com/nature/journal/v520/n7546/full/nature14299.html

