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BioAssay Systems/QuantiChrom™ Boron Assay Kit/100 tests/DBOR-100
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BioAssay Systems/QuantiChrom™ Boron Assay Kit/100 tests/DBOR-100
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BioAssay Systems
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DBOR-100
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QuantiChrom™ Boron Assay Kit

QuantiChrom™ Boron Assay Kit Catalog No: DBOR-100
Price: $279 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: RT Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: RT
Boron Assay Kit
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ProtocolSDS

Application

  • Quantitative determination of boron in agricultural and environmental samples.

Key Features

  • Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well).
  • Convenient. The procedure involves adding a single working reagent.
  • High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.

Method

  • OD420nm

Samples

  • Water, plant tisse, soil samples, and antibody conjugation solutions.

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.05 μg/mL or 0.05 ppm

Shelf Life

  • 12 months

More Details

  • BORON is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes, and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount of boron available to plants is actually quite small. As a result, boron deficiency is the second most common micronutrient deficiency among crop plants. In order to keep plant boron levels in a healthy range, supplementation to the soil via fertilizers and additives is often required. If not regulated, a lack of or excess of boron may significantly lower crop yield. In the biotech industry, sodium borohydride is commonly used to conjugate antibodies and typically needs to be removed from the final product, especially for therapeutic antibodies.BioAssay Systems' boron detection kit provides a convenient and reliable means to measure boron. In the assay borate complexes with azomethine-H to create a colored compound that can be measured at 420 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.

My sample has background absorbance at OD420nm; will this be an issue with the assay?

The calculation provided in the protocol accounts for sample background by subtracting the sample background blank.

After I make the working reagent, how long is it good for?

The working reagent should be made fresh and used within 15 minutes.

My samples have very high/low ODs, what is happening?

If your samples have very low ODs you may be below the kit detection limit. If your sample is from a soil or plant extraction, consider a more concentrated extraction to increase the amount of boron in the sample. If your samples have high ODs consider diluting them so they fall within the linear range of the standard curve.

Can I use this kit to determine the level of boron in my metal, glass, etc.?

This kit is optimized to detect boron in the form of borates and cannot be guaranteed to work with synthesized, and/or exotic boron compounds. However, samples may be treated to convert boron to borates.

How can I extract boron from a soil sample?

For dry weight analysis, first completely dry the soil to remove all water weight. Next, we recommend mixing 5 g of soil (sieved to remove rocks and debris) with 50 mL of 50 mM HCl for 30 min. Centrifuge the sample to pellet precipitates and use the supernatant for the assay. Note: if there are soil particles floating make sure to avoid them when pipetting; your sample should be completely free of particulate matter.

How can I extract boron from a plant sample?

For dry weight analysis, first completely dry the plant matter to remove all water weight. Next, we recommend burning 5 to 10 g of plant matter to ashes in a crucible at 500 degrees Fahrenheit for 3 hours in a drying oven. You may also use a blow torch to burn to ashes if a drying oven is not available. Mix all of the ashed plant material with 50 mL of 50 mM HCl for 30 min. Centrifuge the sample to pellet the ash and use the supernatant for the assay. Note: if there are ash particles floating make sure to avoid them when pipetting; your sample should be completely free of particulate matter.

My sample is in an acidic/basic solution, can I use it for the assay?

If samples are strongly acidic or basic they should be adjusted to a pH of 5-7. Weak or dilute acids and bases do not need to be pH adjusted as the Working Reagent has sufficient buffering capacity.

Why do you subtract the water OD from the background OD in the formula?

In the [Boron] equation the ODBLANK is subtracted from the ODSAMPLE to remove any contribution from the reagent, plate, and reaction volume from the OD of the sample. ODH2O is subtracted from the ODSAMPLE BG so that only the background absorbance of the sample is further subtracted from the ODSAMPLE or, in other words, to make sure that the plate and reaction volume contributions to the ODSAMPLE are not subtracted out twice.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
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DNA提取与纯化123
山中树2021-07-20
我想从植物中提取总DNA做酶切来扩增启动子,但是不论怎么纯化,用平末端的酶老是切不动,请问各位大虾有没有什么高见啊?!对了,我用的是CTAB法,在用无水乙醇沉淀之后不离心直接用枪头把DNA挑出来了洗了,但是还是切不动.好烦啊,请教各位大虾了!
rn2502试剂盒提取rna还需要哪些试剂
准备RNA用的试剂:70%乙醇(用DEPC处理后的水稀释新开封的无水乙醇),DEPC处理后的水,新开封的三氯甲烷等,
容器:玻璃试剂瓶需要180度,8个小时以上烘烤
Eppendorf管和“枪头”:RNase-free的管子和“枪头”(可以直接买到);或者普通的ep管和“枪头”,但是需要DEPC水浸泡后,高温灭菌(121度,20-40分钟)。
如果需要使用研钵和药品匙,研钵和药品匙也是需要180度,8小时以上的烘烤。
还需要没开封的手套,提取RNA时,尽量勤换手套,少说话,尽量不要对着样品吹气。
CTAB法提取DNA中各试剂的用途
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.
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请问各位大神,我实验室有这些试剂盒,QIAprepSpinMiniprepKit(250)、AllPrepDNA/RNAMiniKit(50)、QIAampDNAMiniKit、WizardGenomicDNAPurificationKit,请问这几个提DNA的试剂盒有什么不同吗?
有哪些可以提取细胞中病毒DNA的?或者用其他试剂盒提过呢
坐等回复!
水稻和棉花的种子
dna提取试剂盒说明书123
ewen71172021-07-23
**推荐性价比高的DNA提取试剂盒,提取分选后的细胞,细胞量不大。
天根RNA试剂盒DP419提取的蛋白可以做WB吗:分三层后,上层是RNA,那个中间层的蛋白怎么提出来纯化一下做WB?请高手解答,非常感谢!
这两个方法有什么区别?那个比较好?有什么要主意的吗?

提取试剂盒的详细步骤是什么?
RNA干扰的成功与困惑123
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http://www.bioon.com/biology/Special/RNAi/Index.shtml
这里有关于miRNA专题报道,可以看看
想请问各位大神~哪个厂家的真菌RNA提取的试剂盒性价比高点呀~~本人毕业论文,想做基因表达的~~查了一下天根RNAprepPure植物总RNA提取试剂盒,跟生工的酵母总RNA快速抽提试剂盒与真菌总RNA快速抽提试剂盒,就想在这三个里面选~~但天根的比较贵呀~~生工的效果有不知道如何~?想问一下各位有木有用过这几个试剂盒的?那样好一点呀~~求回复~~~~谢谢各位了~
我在提一种植物的RNA用的是上海华舜生物公司的TRIZOL按照他上面的说明书,我应该用System4那种方法,可是里面要用到RNA沉淀试剂,不知道是什么东西,试剂盒只有一瓶,是RNAexReagent。其它的就没有了。不知道那东西是什么,要自己配吗?RNA沉淀试剂是什么东西?