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BioAssay Systems/QuantiChrom™ Boron Assay Kit/100 tests/DBOR-100
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4000-520-616
QuantiChrom™ Boron Assay Kit
- Product Overview
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ProtocolSDS
Application
- Quantitative determination of boron in agricultural and environmental samples.
Key Features
- Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well).
- Convenient. The procedure involves adding a single working reagent.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.
Method
- OD420nm
Samples
- Water, plant tisse, soil samples, and antibody conjugation solutions.
Species
- All
Size
- 100 tests
Detection Limit
- 0.05 μg/mL or 0.05 ppm
Shelf Life
- 12 months
More Details
- BORON is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes, and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount of boron available to plants is actually quite small. As a result, boron deficiency is the second most common micronutrient deficiency among crop plants. In order to keep plant boron levels in a healthy range, supplementation to the soil via fertilizers and additives is often required. If not regulated, a lack of or excess of boron may significantly lower crop yield. In the biotech industry, sodium borohydride is commonly used to conjugate antibodies and typically needs to be removed from the final product, especially for therapeutic antibodies.BioAssay Systems' boron detection kit provides a convenient and reliable means to measure boron. In the assay borate complexes with azomethine-H to create a colored compound that can be measured at 420 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.
My sample has background absorbance at OD420nm; will this be an issue with the assay?
The calculation provided in the protocol accounts for sample background by subtracting the sample background blank.After I make the working reagent, how long is it good for?
The working reagent should be made fresh and used within 15 minutes.My samples have very high/low ODs, what is happening?
If your samples have very low ODs you may be below the kit detection limit. If your sample is from a soil or plant extraction, consider a more concentrated extraction to increase the amount of boron in the sample. If your samples have high ODs consider diluting them so they fall within the linear range of the standard curve.Can I use this kit to determine the level of boron in my metal, glass, etc.?
This kit is optimized to detect boron in the form of borates and cannot be guaranteed to work with synthesized, and/or exotic boron compounds. However, samples may be treated to convert boron to borates.How can I extract boron from a soil sample?
For dry weight analysis, first completely dry the soil to remove all water weight. Next, we recommend mixing 5 g of soil (sieved to remove rocks and debris) with 50 mL of 50 mM HCl for 30 min. Centrifuge the sample to pellet precipitates and use the supernatant for the assay. Note: if there are soil particles floating make sure to avoid them when pipetting; your sample should be completely free of particulate matter.How can I extract boron from a plant sample?
For dry weight analysis, first completely dry the plant matter to remove all water weight. Next, we recommend burning 5 to 10 g of plant matter to ashes in a crucible at 500 degrees Fahrenheit for 3 hours in a drying oven. You may also use a blow torch to burn to ashes if a drying oven is not available. Mix all of the ashed plant material with 50 mL of 50 mM HCl for 30 min. Centrifuge the sample to pellet the ash and use the supernatant for the assay. Note: if there are ash particles floating make sure to avoid them when pipetting; your sample should be completely free of particulate matter.My sample is in an acidic/basic solution, can I use it for the assay?
If samples are strongly acidic or basic they should be adjusted to a pH of 5-7. Weak or dilute acids and bases do not need to be pH adjusted as the Working Reagent has sufficient buffering capacity.Why do you subtract the water OD from the background OD in the formula?
In the [Boron] equation the ODBLANK is subtracted from the ODSAMPLE to remove any contribution from the reagent, plate, and reaction volume from the OD of the sample. ODH2O is subtracted from the ODSAMPLE BG so that only the background absorbance of the sample is further subtracted from the ODSAMPLE or, in other words, to make sure that the plate and reaction volume contributions to the ODSAMPLE are not subtracted out twice.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
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蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
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Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
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商品咨询
DNA提取过程中各试剂的作用？需要各个试剂的详细作用,谢谢_123
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需要什么试剂？具体的配制方法是怎样的？要非常具体的！
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求水煮法提取DNA的详细过程，复制他人的请注明来源网址，谢谢
不懂得请别粘贴那些有的没的，浪费资源
采用的给全部分
不懂得请别粘贴那些有的没的，浪费资源
采用的给全部分
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http://www.bioon.com/biology/Special/RNAi/Index.shtml
这里有关于miRNA专题报道，可以看看
这里有关于miRNA专题报道，可以看看
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题目错误
如何进行动物体内RNA干扰实验? 实验动物与组织学技术讨论版...123
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动物体内RNA转染试剂，核酸和转染试剂混合后注射，轻松进行RNA干扰，基因敲除、沉默实验，3天可得出结果。下面以50μg的核酸与25μl转染试剂，总注射体积200μl，20g小鼠尾静脉注射为例说明。局部注射不用稀释，直接根据需要把核酸和转染试剂混合即可使用。
1.核酸的稀释。将50μg核酸用适量无内毒素纯水稀释成1μg/μl，加入10%葡萄糖溶液(w/v)50μl，使葡萄糖终浓度为5%，终体积为100μl，充分混匀。
2.转染试剂的稀释。取25μl的Entranster-invivo试剂用50μl的10%葡萄糖溶液稀释，并用纯水25μl补足，得到葡萄糖终浓度为5%，终体积为100μl液体，充分混匀。
3.转染复合物形成。立即将稀释后的转染试剂加入到稀释后的核酸溶液中，立即充分振荡混匀。
4.室温静置15分钟。配制好的转染复合物请即配即用，不宜长期存放。
5.动物注射。
说明：1).尾静脉注射时请掌握注射技巧，一般选用远端1/3处静脉注射，如感觉到阻力和轻微隆起，请停止注射，重新寻找静脉进行操作，不要强力推注，否则容易将药液注射在尾部，导致尾部溃烂。注射完成后移去针头，按压针孔10秒以上，防止药液流出。2).2.5mg/kg的给药剂量是起始给药剂量，如果动物可以耐受，可以按比例增加剂量，这样效果更好。3).局部注射，尽可能多注射药液，有利于提高转染效果。
6.基因表达检测。一般来说，根据注射方法和靶器官的差异，12-48小时后基因表达效果较好。
7.长期给药。一次给药检测的最佳时间是注射后12-48小时。如果需要维持长期效果，可以采用多次注射的方法，注射频度一般为每间隔2-3天一次，也可以根据实验情况适当延长至每7天一次。
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我在提一种植物的RNA用的是上海华舜生物公司的TRIZOL按照他上面的说明书，我应该用System4那种方法，可是里面要用到RNA沉淀试剂，不知道是什么东西，试剂盒只有一瓶，是RNAexReagent。其它的就没有了。不知道那东西是什么，要自己配吗？RNA沉淀试剂是什么东西？
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