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mRNA PurificationI. Prepare oligo-dT celluloseuse 40 mg oligo-dT cellulose / 1 mg total RNA swell oligo dT-cellulose in elution buffer wash oligo dT-cellulose
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PolyATtract® mRNA Isolation SystemsThe PolyATtract® mRNA Isolation Systems utilize Promega"s MagneSphere® technology to eliminate the need for olig
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Nucleotide Composition of RNALEVEL II Materials RNA sample1 N and 0.1 N HClBoiling water bathWhatman #1 filter paper (for chromatography)Chromatography tank20
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When isolating RNA, it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells. It is also benefici
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Sucrose Density FractionationLEVEL II Figure 14.2 Sucrose gradient distribution of RNA Materials 10% and 40% (w/v) Sucrose 80.02 M sodium acetate, pH 5.1, cont
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Spectrophotometric Analysis of rRNALEVEL I Materials RNAUV spectrophotometer and cuvettesAlkaline distilled water Procedure Dissolve 10 mg of commercial RNA in
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Phenol Extraction of rRNA (Rat liver)LEVEL II *** READ THROUGH ALL CAUTIONS BEFORE TRYING THIS EXPERIMENT *** Materials Rat liver (fasted rat)Liquid nitrogenp-
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N.B: Care must be taken when handling solutions containing high concentrations of guanidine salts due to its chaotropic nature. As with other procedures involv
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DROSOPHILA RNA PREP(Goodman Lab) Stock Solutions 3M NaOAc, pH 5.2 with HAc (MW=82) 6M Guanidine Hydrochloride in 0.1M NaOAc, pH 5.2 (MWGuHCl=95.54) 5.7M Cesium
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