DescriptionThe GF-1 Blood DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from fresh and frozen anti-coagulated whole blood. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.
Features
- Yields up to 20μg of DNA
- No organic-based extraction required
- Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.
Kit Components
- Buffer BB
- Wash Buffer 1 (concentrate)
- Wash Buffer 2 (concentrate)
- Elution Buffer
- Proteinase K
Ordering Information
| Catalog No | Description | Pack Size |
| GF-BD-050 | GF-1 Blood DNA Extraction Kit | 50 preps |
| GF-BD-100 | GF-1 Blood DNA Extraction Kit | 100 preps |
DownloadManual
GF-1 Blood DNA Extraction Kit
Stability Test Report
GF-1 Blood DNA Extraction Kit
PublicationThis Product Has Been Used In: Alhomsi et al. (2020) Assessment of vitamin D-binding protein (DBP) gene polymorphisms and their correlation with multiple sclerosis: a case-control study in a sample of the Syrian population, Egyptian Journal of Medical Human Genetics, 21:32Gorgisen G, Karatas U, Ates C, Oksuz M & Gulacar IM (2020)Association of IRS1 Gly972Arg and IRS2 Gly1057Asp polymorphisms with gastric cancer in Turkish subjects, Oncology Letters, 20:2016-2020Wannapa, Settheetham-Ishida et al. (2020)Genetic Polymorphism of Glutathione S-transferase and Cervical Cancer Susceptibility in Northeastern Thailand, Asian Pacific Journal of Cancer Biology, 5(2): 35-41.Wongpratate et al. (2020)Genetic Polymorphisms of the Human Cytochrome P450 1A1 (CYP1A1) and Cervical Cancer Susceptibility among Northeast Thai Women, Asian Pacific Journal of Cancer Prevention, 21(1):243-248.Yusuf et al. (2020) A preliminary study MUC5B promoter polymorphism and its association with IPF, The Egyptian Journal of Bronchology, 14:18.Panan Kanchanaphum (2018) Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction, . BioMed Research International.Cilingir, O, Ozkan S., Aras, B.D., Erzurumluoglu, E., Kutlay, O., Akinci, M., Emir, B., Afagh, A., Artan, S., (2017) Association of functional RAGE gene polymorphisms with Parkinson’s disease in a Turkish cohort. Biomedical Research 2017; 28 (19): 8454-8460Piratae, S., Sae-chue, B., Sukumolanan, P., Phosri, A. (2017).). Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand, . , Asian Pacific Journal of Tropical Disease , Vol. 7, No. 4, 233-236 (2017).. Rani, A., Nawaz, S.K., Irfan, S., Arshad, M., Bashir, R., Shaheen, N. (2017). Role of MyD88-adaptor-like gene polymorphism rs8177374 in modulation of malaria severity in the Pakistani population, The Brazilian Journal of Infectious Diseases, Vol. 21, No. 4. (2017). Abajy, M.Y., Ibrahim, A., Almohsen, J.A.(2016). Development of New AS-PCR based Analytical Approach for detecting the Single Nucleotide Polymorphism of AGTR.1 gene, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, No. 7 (2016). Babker,A.M.A.A., Gameel, F.E.M.H. (2016).Methylenetetrahydrofolate Reductase C677T Polymorphism in Sudanese Women with Recurrent Spontaneous Abortions, Kuwait Medical Journal, Vol. 48, No. 2, 100-104 (2016).Zahri, M.K., Emilia, A., Rawi, R.I.M., Taib W.R.W., Sani, A.I., Baig, A.A. (2016)Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity. Meta Gene. 10. Pp..39-44. Nawaz, S.K., et al. (2015) Role of S180L Polymorphism in Etiology of Malaria Caused by Plasmodium falciparum in a Small Group of Pakistani Population. Bosnian Journal of Basic Medical Sciences. 15(4), p.20-23. Nawaz, S.K., Rani, A., Yousaf, M., Noreen, A., Arshad, M. 2015. Genetic etiology of coronary artery disease considering NOS 3 genevariant rs1799983. Vascular. 23(3) pp.270-276Naila, R., et al (2013)Elevated genetic deletion of GSTT1 in Pakistani population JÖKULL Journal63(12).Skalar, C., Gurbuz, E., Kalay, N., Kaya, M.G.(2013). Higher frequency of 4977574 (the G Allele) on Chromosome 9p21.3 in Patients with Myocardial Infarction as Revealed by PCR-RFLP Analysis (2013) The Tohoku Journal of Experimental Medicine, Vol 230, No. 3, 171-176 (2013).Amer, H.M. et al. (2011) An Optimized Polymerase Chain Reaction Assay to Identify Avian Virus Vaccine Contamination with Chicken anemia Virus Journal of Veterinary Diagnostic Investigation. Sage Journals. 23, p. 34-40.
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总RNA提取试剂盒（TRIzol法）
RIpure试剂是直接从细胞或组织中提取总RNA的试剂。它在破碎和溶解细胞时能保持RNA的完整性。加入氯仿后离心，样品分成水样层和有机层。RNA存在于水样层中。收集上面的的水样层后，RNA可以通过异丙醇沉淀来还原。在除去水样层后，样品中的DNA和蛋白也能相继以沉淀的方式还原。乙醇沉淀能析出中间层的DNA，在有机层中加入异丙醇能沉淀出蛋白。共纯化DNA对于样品间标准化RNA的产量十分有用。
无论是人、动物、植物还是细菌组织，该方法对少量的组织(50-100mg)和细胞(5×106)以及大量的组织(≥1g)和细胞(>107)均有较好的分离效果。TRIPURE试剂操作上的简单性允许同时处理多个的样品。所有的操作可以在一小时内完成。TRIPURE抽提的总RNA能够避免DNA和蛋白的污染。故而能够作RNA印迹分析、斑点杂交、poly(A)+选择、体外翻译、RNA酶保护分析和分子克隆。如果是用于PCR，当两条引物位于单一外显子内时，建议用扩增级的DNase I来处理抽提的总RNA。
TRIpure试剂能促进不同种属不同分子量大小的多种RNA的析出。例如，从大鼠肝脏抽提的RNA琼脂糖凝胶电泳并用溴化乙啶染色，可见许多介于7 kb和15 kb之间不连续的高分子量条带(mRNA和hnRNA成分)，两条优势核糖体~5 kb (28S)和~2 kb(18S)，低分子量RNA介于0.1和0.3 kb之间 (tRNA, 5S)。当抽提的RNA用TE稀释时其A260/A280比值≥1.8。
1.破碎和裂解植物细胞壁。
2.抑制rna酶
3.去除植物多糖和多酚。树脂 淀粉和纤维材料。
4.去除许多高等植物组织尤其是成熟组织能产生某些水溶性的次级代谢产物，这些次级代谢产物很容易与RNA结合并与RNA共同被抽提出来而阻碍具有生物活性的RNA分离。
除了干扰RNA分离之外，植物多糖和多酚及次级代谢产物还能明显抑制下游RNA反应如转录和PCR.
－－本来，看好丹麦Exiqon公司，Qiagen的两个试剂盒，无奈经费有限，这两个盒子很不错，价格也不菲，有米的兄弟姐妹可以买。
－－另外查询到国产上海诺伦公司的血液(血清/血浆)总RNA抽提试剂盒－－广州这边用的人不多，不知道上海那边的兄弟姐妹有什么经验？
－－－还有，北京康为世纪公司的游离RNA（血清血浆尿液）提取试剂，货号：CW2281，也比较便宜，但是俺心中也没有底，请大家给点意见。
－－－已经订购了LIFETECHNOLOGY的TrizolLS专门提取体液RNA的试剂（100毫升2000元人民币，也不菲），准备预试验看看提取RNA的质量。(Lifetechnology公司的AM1556也不错，但是也不便宜，每个盒子40次/2590元）
－－－－但是还是希望有试剂盒，比较简单，快捷，最重要的是样本量太大，要分离400份左右的血清。
求助：最近要提取血浆中的RNA，不知道用哪种试剂好，查了好多资料，有用Trizol，TrizolLS，RNAisoBlood和天根。但我从没做过，求各位大神给点意见。提取的RNA用于qPCR。
准备RNA用的试剂：70%乙醇（用DEPC处理后的水稀释新开封的无水乙醇），DEPC处理后的水，新开封的三氯甲烷等，
容器：玻璃试剂瓶需要180度，8个小时以上烘烤
Eppendorf管和“枪头”：RNase-free的管子和“枪头”（可以直接买到）；或者普通的ep管和“枪头”，但是需要DEPC水浸泡后，高温灭菌（121度，20-40分钟）。
如果需要使用研钵和药品匙，研钵和药品匙也是需要180度，8小时以上的烘烤。
还需要没开封的手套，提取RNA时，尽量勤换手套，少说话，尽量不要对着样品吹气。
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氯仿萃取RNA
异丙醇沉淀RNA
酒精清洗
