DescriptionThe GF-1 Blood DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from fresh and frozen anti-coagulated whole blood. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.
Features
- Yields up to 20μg of DNA
- No organic-based extraction required
- Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.
Kit Components
- Buffer BB
- Wash Buffer 1 (concentrate)
- Wash Buffer 2 (concentrate)
- Elution Buffer
- Proteinase K
Ordering Information
| Catalog No | Description | Pack Size |
| GF-BD-050 | GF-1 Blood DNA Extraction Kit | 50 preps |
| GF-BD-100 | GF-1 Blood DNA Extraction Kit | 100 preps |
DownloadManual
GF-1 Blood DNA Extraction Kit
Stability Test Report
GF-1 Blood DNA Extraction Kit
PublicationThis Product Has Been Used In: Alhomsi et al. (2020) Assessment of vitamin D-binding protein (DBP) gene polymorphisms and their correlation with multiple sclerosis: a case-control study in a sample of the Syrian population, Egyptian Journal of Medical Human Genetics, 21:32Gorgisen G, Karatas U, Ates C, Oksuz M & Gulacar IM (2020)Association of IRS1 Gly972Arg and IRS2 Gly1057Asp polymorphisms with gastric cancer in Turkish subjects, Oncology Letters, 20:2016-2020Wannapa, Settheetham-Ishida et al. (2020)Genetic Polymorphism of Glutathione S-transferase and Cervical Cancer Susceptibility in Northeastern Thailand, Asian Pacific Journal of Cancer Biology, 5(2): 35-41.Wongpratate et al. (2020)Genetic Polymorphisms of the Human Cytochrome P450 1A1 (CYP1A1) and Cervical Cancer Susceptibility among Northeast Thai Women, Asian Pacific Journal of Cancer Prevention, 21(1):243-248.Yusuf et al. (2020) A preliminary study MUC5B promoter polymorphism and its association with IPF, The Egyptian Journal of Bronchology, 14:18.Panan Kanchanaphum (2018) Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction, . BioMed Research International.Cilingir, O, Ozkan S., Aras, B.D., Erzurumluoglu, E., Kutlay, O., Akinci, M., Emir, B., Afagh, A., Artan, S., (2017) Association of functional RAGE gene polymorphisms with Parkinson’s disease in a Turkish cohort. Biomedical Research 2017; 28 (19): 8454-8460Piratae, S., Sae-chue, B., Sukumolanan, P., Phosri, A. (2017).). Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand, . , Asian Pacific Journal of Tropical Disease , Vol. 7, No. 4, 233-236 (2017).. Rani, A., Nawaz, S.K., Irfan, S., Arshad, M., Bashir, R., Shaheen, N. (2017). Role of MyD88-adaptor-like gene polymorphism rs8177374 in modulation of malaria severity in the Pakistani population, The Brazilian Journal of Infectious Diseases, Vol. 21, No. 4. (2017). Abajy, M.Y., Ibrahim, A., Almohsen, J.A.(2016). Development of New AS-PCR based Analytical Approach for detecting the Single Nucleotide Polymorphism of AGTR.1 gene, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, No. 7 (2016). Babker,A.M.A.A., Gameel, F.E.M.H. (2016).Methylenetetrahydrofolate Reductase C677T Polymorphism in Sudanese Women with Recurrent Spontaneous Abortions, Kuwait Medical Journal, Vol. 48, No. 2, 100-104 (2016).Zahri, M.K., Emilia, A., Rawi, R.I.M., Taib W.R.W., Sani, A.I., Baig, A.A. (2016)Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity. Meta Gene. 10. Pp..39-44. Nawaz, S.K., et al. (2015) Role of S180L Polymorphism in Etiology of Malaria Caused by Plasmodium falciparum in a Small Group of Pakistani Population. Bosnian Journal of Basic Medical Sciences. 15(4), p.20-23. Nawaz, S.K., Rani, A., Yousaf, M., Noreen, A., Arshad, M. 2015. Genetic etiology of coronary artery disease considering NOS 3 genevariant rs1799983. Vascular. 23(3) pp.270-276Naila, R., et al (2013)Elevated genetic deletion of GSTT1 in Pakistani population JÖKULL Journal63(12).Skalar, C., Gurbuz, E., Kalay, N., Kaya, M.G.(2013). Higher frequency of 4977574 (the G Allele) on Chromosome 9p21.3 in Patients with Myocardial Infarction as Revealed by PCR-RFLP Analysis (2013) The Tohoku Journal of Experimental Medicine, Vol 230, No. 3, 171-176 (2013).Amer, H.M. et al. (2011) An Optimized Polymerase Chain Reaction Assay to Identify Avian Virus Vaccine Contamination with Chicken anemia Virus Journal of Veterinary Diagnostic Investigation. Sage Journals. 23, p. 34-40.
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氯仿萃取RNA
异丙醇沉淀RNA
酒精清洗
－－本来，看好丹麦Exiqon公司，Qiagen的两个试剂盒，无奈经费有限，这两个盒子很不错，价格也不菲，有米的兄弟姐妹可以买。
－－另外查询到国产上海诺伦公司的血液(血清/血浆)总RNA抽提试剂盒－－广州这边用的人不多，不知道上海那边的兄弟姐妹有什么经验？
－－－还有，北京康为世纪公司的游离RNA（血清血浆尿液）提取试剂，货号：CW2281，也比较便宜，但是俺心中也没有底，请大家给点意见。
－－－已经订购了LIFETECHNOLOGY的TrizolLS专门提取体液RNA的试剂（100毫升2000元人民币，也不菲），准备预试验看看提取RNA的质量。(Lifetechnology公司的AM1556也不错，但是也不便宜，每个盒子40次/2590元）
－－－－但是还是希望有试剂盒，比较简单，快捷，最重要的是样本量太大，要分离400份左右的血清。
TRIZOL的主要成分是苯酚。苯酚的主要作用是裂解细胞，使细胞中的蛋白，核酸物质解聚得到释放。苯酚虽可有效地变性蛋白质，但不能完全抑制RNA酶活性，因此TRIzol中还加入了8－羟基喹啉、异硫氰酸胍、β-巯基乙醇等来抑制内源和外源RNase（RNA酶）。
TRIZOL是从细胞和组织中提取总RNA的即用型试剂，在样品裂解或匀浆过程中，TRIZOL 能保持RNA完整性。加入氯仿后，溶液分为水相和有机相，RNA在水相中。取出水相，用异丙醇可沉淀回收RNA。
※0.1%的8－羟基喹啉可以抑制RNase，与氯仿联合使用可增强抑制作用。
※异硫氰酸胍属于解偶剂，是一类强力的蛋白质变性剂，可溶解蛋白质并使蛋白质二级结构消失，导致细胞结构降解，核蛋白迅速与核酸分离。
※β-巯基乙醇的主要作用是破坏RNase蛋白质中的二硫键。
TRIZOL是一种新型总RNA抽提试剂，可以直接从细胞或组织中提取总RNA。其含有苯酚、异硫氰酸胍等物质，能迅速破碎细胞并抑制细胞释放出的核酸酶。TRIZOL在破碎和溶解细胞时能保持RNA的完整性，因此对纯化RNA及标准化RNA的生产十分有用。
基本特点
Trizol试剂可以快速提取人、动物、植物、细菌不同组织的总RNA，该方法对少量的组织（50-100 mg）和细胞（5×106）以及大量的组织（≥1 g）和细胞（>107）均有较好的分离效果。TRIZOL试剂操作上的简单性允许同时处理多个的样品。所有的操作可以在一小时内完成。TRIZOL抽提的总RNA能够避免DNA和蛋白的污染。故而能够作RNA 印迹分析、斑点杂交、poly(A)+ 选择、体外翻译、RNA酶保护分析和分子克隆。并且利用DNA、RNA和蛋白质在不同溶液中的溶解性质，可以通过分层分别将不同层中的RNA（上层）、DNA（中层）、蛋白质（下层）分离纯化出来，效率极好。
Trizol试剂能促进不同种属不同分子量大小的多种RNA的析出。例如，从大鼠肝脏抽提的RNA琼脂糖凝胶电泳并用溴化乙啶染色，可见许多介于7 kb和15 kb之间不连续的高分子量条带，（mRNA和hnRNA成分）两条优势核糖体RNA条带位于~5 kb （28S）和~2 kb （18S），低分子量RNA介于0.1 和 0.3 kb之间 (tRNA,5S）。当抽提的RNA用TE稀释时其A260/A280比值≥1.8
TRIZOL
http://baike.baidu.com/view/533842.htm
Trizol法和试剂盒提取组织中的RNA，哪个方法更可靠和精确呢？谢谢！
这里有关于miRNA专题报道，可以看看
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