
Overview:
PDE10A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP (1). PDE10A eliminates cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate. PDE10A hydrolyzes cAMP and cGMP in transfected COS-7 cells (2). PDE10A has a 11-fold higher affinity for cGMP than cAMP and cAMP can efficiently inhibit cGMP phosphodiesterase activity of PDE10A. PDE10A is being used as a biomarker in clinical trials of gene-disease association and gene-environment interaction.
Gene Aliases:
HSPDE10A; FLJ11894; FLJ25677
Genbank Number:
NM_006661
References:
1. Fujishige, K. et.al: Cloning and characterization of a novel human phosphodiesterase that hydrolyzes both cAMP and cGMP (PDE10A). J. Biol. Chem. 274: 18438-18445, 1999.2. Loughney, K et.al: Isolation and characterization of PDE10A, a novel human 3-prime, 5-prime-cyclic nucleotide phosphodiesterase. Gene 234: 109-117, 1999.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

