Overview:
Diacylglycerol kinase theta (DAG kinase theta) is the only member of class V diacylglycerol kinase, which contains three cysteine-rich domains, a proline-rich region, and a Ras-associating domain and mediates the regeneration of phosphatidylinositol from diacylglycerol in cell signal transduction (1). DGKQ has been associated with Parkinson’s disease in a genome-wide meta-analysis study (2). Stimulated DGK theta activity promotes the SF1-dependent transcription of CYP17 in human adrenocortical cells (3).
Gene Aliases:
DAGK; DAGK4; DAGK7
Genbank Number:
NM_001347
References:
1. Houssa, B. et al. Cloning of a Novel Human Diacylglycerol Kinase (DGK theta) Containing Three Cysteine-rich Domains, a Proline-rich Region, and a Pleckstrin Homology Domain with an Overlapping Ras-associating Domain. The Journal of Biological Chemistry. (272) 10422–10428, 1997.2. Nalls, M. et al. Large-scale meta-analysis of genome-wide association data identifies six new risk loci for Parkinson’s disease. Nature Genetics. (46) 989-993, 2014.3. Li, Donghui. et al. Cyclic AMP-Stimulated Interaction between Steroidogenic Factor 1 and Diacylglycerol Kinase theta Facilitates Induction of CYP17. Molecular and Cellular Biology. (27) 6669–6685, 2007.
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最近遇到了实验上的大问题，就是用一步法做RNA的荧光定量PCR时，发现低浓度的做不出来，而DNA是可以的，想请问各位在一步法PCR中有没有什么高见，就是逆转录的Buffer有没有什么需要注意的地方！就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer！
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内（外）起作用消化酶细胞外起作用B错误；
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误；
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确．
故选：D．
我是新手。我要开始做实验了，但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒，向好几家公司打了电话，但都没有我想要的东西。
没有这个东西，我的实验就只能是蓝图了。哪位战友有办法的，能不能帮帮我？
不胜感激。
请规范发贴，求助贴请在标题前加【求助】，谢谢您的合作。——by草根
