
Overview:
CDKL2 or cyclin-dependent kinase-like 2 is a member of a large family of CDC2-related serine/threonine protein kinases that is postnatally expressed in various brain regions. The extremely high CDKL2 expression in brain regions suggests that it has a role in cognition and emotion. CDKL2 transcripts increase in the deep cerebellar nuclei of the rabbit after eyeblink conditioning, a model of associative learning and memory CDKL2, also known as p56 KKIAMRE, accumulates primarily in the cytoplasm with lower levels in the nucleus (1). CDKL2 or p56, and the related protein kinase had a similar pattern of mRNA expression except in the reproductive organs. CDKL2 is expressed in several human tissues, with the highest expression in testis and kidney (2).
Gene Aliases:
KKIAMRE; P56
Genbank Number:
NM_003949
References:
1. Meyerson, M.et.al: A family of human cdc2-related protein kinases. EMBO J. 11: 2909-2917, 1992. 2. Taglienti, C. A. et.al: Molecular cloning of the epidermal growth factor-stimulated protein kinase p56 KKIAMRE. Oncogene 13: 2563-2574, 1996
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

