
Overview:
DNMT1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1). DNMT1 enzyme is crucial for stable expression and function in vivo (2). DNMT1 is essential for maintenance of DNA methylation and the interaction of DNMT1 with the replication machinery is not strictly necessary for maintenance of DNA methylation, but improves its efficiency. DNMT1 is necessary and sufficient to maintain global methylation and aberrant CpG island methylation in human cancer cells.
Gene Aliases:
Dnmt; MCMT; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; Met1; MommeD2
Genbank Number:
BC053047
References:
1. Yoder, J. A. et.al: New 5-prime regions of the murine and human genes for DNA (cytosine-5)-methyltransferase. J. Biol. Chem. 271: 31092-31097, 1996. 2. Tucker, K. L. et.al: Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene. Proc. Nat. Acad. Sci. 93: 12920-12925, 1996.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

