
Overview:
DRAK1 (STK17A) is a member of the DAP kinase-related apoptosis-inducing protein kinase family. DRAK1 encodes an autophosphorylated nuclear protein with a protein kinase domain which has apoptosis-inducing activity. DRAK1 is capable of autophosphorylation and of phosphorylating myosin light chain as an exogenous substrate. The noncatalytic C terminus of DRAK1 is crucial for full kinase activity (1). DRAK1 is highly expressed in placenta, but also in heart, lung, skeletal muscle, kidney, and pancreas. DRAK1 act as a novel direct target of p53 and a modulator of cisplatin toxicity and reactive oxygen species in testicular cancer cells (2).
Gene Aliases:
DRAK1; STK17A
Genbank Number:
NM_004760
References:
1. Sanjo, H. et.al: DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J. Biol. Chem. 273: 29066-29071, 1998. 2. Mao P, et.al: Serine/threonine kinase 17A is a novel p53 target gene and modulator of cisplatin toxicity and reactive oxygen species in testicular cancer cells. J Biol Chem. 2011 Jun 3;286(22):19381-91.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

