
Overview:
HDAC10 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. HDAC10 belongs to class II of the histone deacetylase family that catalyzes the deacetylation of lysine residues in the N-terminal tail of histones and represses transcription in large multiprotein complexes with transcriptional co-repressors (1). Therefore, HDAC10 plays a role in transcriptional repression. HDAC10 interacts with HDAC3 in co-transfected embryonic kidney cells (2). Deletion analysis indicates that both the N- and C-terminal domains of HDAC10 bound HDAC3 independently.
Gene Aliases:
DKFZp761B039; MGC149722
Genbank Number:
NM_032019
References:
1. Fischer, D. D. et.al: Isolation and characterization of a novel class II histone deacetylase, HDAC10. J. Biol. Chem. 277: 6656-6666, 2002. 2. Tong, J. J. et.al: Identification of HDAC10, a novel class II human histone deacetylase containing a leucine-rich domain. Nucleic Acids Res. 30: 1114-1123, 2002.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

