
Overview:
IRAK1 encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. The exposure of HeLa cells or human embryonic kidney cells overexpressing IL1R to IL1 caused rapid association of IRAK with the IL1R complex and phosphorylation of IRAK (1).IRAK1is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus (2).
Gene Aliases:
IRAK, pelle
Genbank Number:
NM_001569
References:
1. Cao, Z.et.al: IRAK: a kinase associated with the interleukin-1 receptor. Science 271: 1128-1131, 1996. 2. Jacob, C. O. et.al: Identification of IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus. Proc. Nat. Acad. Sci. 106: 6256-6261, 2009.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

