
Overview:
MSK2 or mitogen- and stress-activated protein kinase-2 is a member of the serine/threonine kinases family that contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. MSK2 is also known as ribosomal protein S6 kinase 4 (RPS6KA4) that are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38.
Gene Aliases:
RPS6KA4; RSK-B; RSKB
Genbank Number:
BC012964
References:
1. Deak, M. et.al: Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17: 4426-4441, 1998.2. Pierrat, B. et.al: RSK-B, a novel ribosomal S6 kinase family member, is a CREB kinase under dominant control of p38-alpha mitogen-activated protein kinase (p38-alpha-MAPK). J. Biol. Chem. 273: 29661-29671, 1998.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

