Overview:
NCK (or non-catalytic region of tyrosine kinase adaptor protein 1) is an adaptor protein that is located in the cytoplasm and involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS (1). NCK is a signaling and transforming protein that contains Src homology 2 and 3 (SH2 and SH3) domains. NCK functions by coupling tyrosine phosphorylation via SH2 domains to downstream effectors through SH3 domains. Furthermore, NCK couples tyrosine phosphorylation guidance signals to cytoskeletal events required for the ipsilateral projections of spinal cord neurons and for normal limb movement (2).
Gene Aliases:
NCK1, MGC12668, NCKalpha
Genbank Number:
NM_006153
References:
1. Park, D. et al: Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP. Mol. Cell. Biol.1992; 12 (12): 5816�23. 2. Fawcett, J. P. et al: Nck adaptor proteins control the organization of neuronal circuits important for walking. Proc. Nat. Acad. Sci. 104: 20973-20978, 2007.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根
1970科家致癌RNA病毒发现种特殊RNA病毒聚合酶,该酶能RNA模板,根据碱基互补原则.程与般病毒转录向相反,故称逆转录,催化程酶称逆转录酶,发现哺乳物胚胎细胞裂淋巴细胞含.
逆转录病毒称携带逆转录酶病毒,先侵入宿主细胞病毒RNA模板,靠酶形DNA环化,合宿主细胞染色体原病毒形式宿主细胞代代传.
HIV(艾滋病病毒)典型逆转录病毒
最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
⒈通过嵌入DNA双链的碱基之间,形成稳定复合物,抑制DNA复制与RNA合成,从而阻碍快速生长的癌细胞的分裂。
⒉抑制拓扑异构酶II,影响DNA超螺旋转化成为松弛状态,从而阻碍DNA复制与转录。有研究显示拓扑异构酶II抑制剂(除蒽环类药物还包括依托泊苷等)能够阻止拓扑异构酶II的翻转,而这点对于它从它的核酸底物上脱离是必需的。这就意味着,拓扑异构酶II抑制剂使拓扑异构酶II的复合物在DNA链断裂之后才能更稳定,导致后者催化了DNA的破坏;同时,拓扑异构酶II抑制剂还能阻碍连接酶对DNA的修复。
⒊螯合铁离子后产生自由基从而破坏DNA、蛋白质及细胞膜结构。向左转|向右转
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.

