
Overview:
p73 gamma is a member of the p53 family of transcription factors which are involved in cellular responses to stress and development. There are four isoforms of p73 (p73 alpha, p73 beta, p73 delta and p73 gamma) which differed in their abilities to form homodimeric and heterodimeric interactions with each other and with p53 (1). The p73 isoforms also differed in their abilities to activate transcription of the p21(Waf1) promoter and to inhibit colony formation. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. p73 is a stress-response gene that activates transcription of p53-responsive genes and inhibits cell growth in a p53-like manner by inducing apoptosis (2).
Gene Aliases:
TP73, TAp73alpha
Genbank Number:
O15350-3
References:
1. De Laurenzi, V. et al: Two new p73 splice variants, gamma and delta, with different transcriptional activity. J. Exp. Med. 188: 1763-1768, 1998. 2. Jost. C. A. et.al: p73 is a human p53-related protein that can induce apoptosis. Nature 389: 191-194, 1997.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

