Overview:
Troponin1 is a member of the tropomyosin family and regulates the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin1 is part of a complex with troponin T and troponin C in which Troponin1 acts as an inhibitory subunit that blocks actin-myosin interactions and thereby mediates striated muscle relaxation (1). Troponin1 is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The Troponin1 protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes (2).
Gene Aliases:
TNNI1; DKFZp451O223; SSTNI; TNN1
Genbank Number:
NM_003281
References:
1. Tiso, N. et al: Fine mapping of five human skeletal muscle genes: alpha-tropomyosin, beta-tropomyosin, troponin-I slow-twitch, troponin-I fast-twitch, and troponin-C fast. Biochem. Biophys. Res. Commun. 230: 347-350, 1997. 2. Nadal-Ginard.et al: Molecular basis of cardiac performance: plasticity of the myocardium generated through protein isoform switches. J. Clin. Invest. 84: 1693-1700,198
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1970科家致癌RNA病毒发现种特殊RNA病毒聚合酶,该酶能RNA模板,根据碱基互补原则.程与般病毒转录向相反,故称逆转录,催化程酶称逆转录酶,发现哺乳物胚胎细胞裂淋巴细胞含.
逆转录病毒称携带逆转录酶病毒,先侵入宿主细胞病毒RNA模板,靠酶形DNA环化,合宿主细胞染色体原病毒形式宿主细胞代代传.
HIV(艾滋病病毒)典型逆转录病毒
⒈通过嵌入DNA双链的碱基之间,形成稳定复合物,抑制DNA复制与RNA合成,从而阻碍快速生长的癌细胞的分裂。
⒉抑制拓扑异构酶II,影响DNA超螺旋转化成为松弛状态,从而阻碍DNA复制与转录。有研究显示拓扑异构酶II抑制剂(除蒽环类药物还包括依托泊苷等)能够阻止拓扑异构酶II的翻转,而这点对于它从它的核酸底物上脱离是必需的。这就意味着,拓扑异构酶II抑制剂使拓扑异构酶II的复合物在DNA链断裂之后才能更稳定,导致后者催化了DNA的破坏;同时,拓扑异构酶II抑制剂还能阻碍连接酶对DNA的修复。
⒊螯合铁离子后产生自由基从而破坏DNA、蛋白质及细胞膜结构。向左转|向右转
最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!