Description
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Transfection Reagent for C6 Cells (Glioma Cells, CCL-107)
- Two component formulation enhances lipid mediated transfection efficiency
- Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
- Kit includes Transfection Enhancer reagent and recommended transfection protocol
- High transfection efficacy in the presence of serum
- Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
- Reproducible transfection results
- Works well for standard reverse transfection and high-throughput applications
- Download in vitro C6 transfection protocol: [PDF]
- Download C6 CRISPR/Cas9 transfection protocol: [PDF]
- Download PowerPoint presentation for C6 cells transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems C6 Transfection Protocol: [PDF]
Download MSDS: [PDF]
C6 Cell Line:
While glioblastoma is not the most common type of brain cancer, it is the most aggressive and deadliest. Glial cells are responsible for providing nourishment and mechanical support to nerve cells in the brain. Gliomas are tumors that originated in glial cells and are characterized based on location. According to the Brain Tumor Center at Johns Hopkins, malignant gliomas comprise about 70% of all new cases of brain tumors and tend to recur despite aggressive combinatorial treatment. New therapies are desperately needed to conquer any potential challenges in the treatment of the malignant brain tumor and improve the survival rate of patients. Research at the cellular level is a helpful tool to gather information on cells that rapidly metastasize such as cancerous glial cells. The C6 glioma cell line was derived from the nervous tissue of a rat (Rattus norvegicus) glial tumor and has proven to be a reliable transfection host for the study of brain tumors. This strain of cells is isolated by N-nitrosomethylurea by Benda et al., exhibiting fibroblast morphology. In addition to being an appropriate host in mammalian brain tumors studies, the C6 cell line is employed for preclinical research worldwide as well as in cellular and molecular biology applications. C6 cells have been known to show glucocorticoid receptors, and they also make for a good transfection research model. Altogen Biosystems provides two-component high-efficiency transfection reagent kits for the C6 glioma cell line.
Data:
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into C6 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in C6 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into C6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
C6 Transfection Reagent
Altogen Biosystems:
Altogen Biosystems provides pre-optimized transfection kits and electroporation products for life sciences and cancer research. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits (animal transfection): 1) Tissue-targeted reagents (delivery of proteins, DNA, and RNA into liver, pancreas, or kidney tissues), and 2) Broad range in vivo biodistribution reagents (PEG-Liposome based reagent, Nanoparticle-based in vivo reagent, Lipid-based transfection kit, and Polymer-based kit). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged biomolecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
Volume Options:
- 0.5 ml (Catalog #6345)
- 1.5 ml (Catalog #6346)
- 1.5 ml CRISPR (Catalog #2121)
- 8.0 ml (Catalog #7026)
AltogenBiosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂、癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNA(mRNA,siRNA,shRNA,microRNA),蛋白质和小分子研究。
Altogen生命科学公司致力于研发,生产和销售特定细胞系的转染试剂,用于细胞间生物分子的传递,并通过对转染试剂类型的设计将siRNA和质粒DNA有效地转入不同的细胞系和原代细胞内。Altogen公司开发的聚合物,脂质体,纳米粒子为基础的转染技术分别针对分子生物学,组合化学,和细胞生物学而分别应用。Altogen定制服务提供符合GLP要求定制研究服务,包括代稳定的细胞系,细胞银行和冷冻保存,焦磷酸测序,克隆,RNA干扰(RNAi)和基因沉默服务,发展分析,siRNA文库筛选,并转染服务。稳定的肿瘤细胞株和原代细胞的产生,可以是非常昂贵和费时。该公司的细胞培养科学家的细胞株的选择,无论是利息或shRNA表达载体的稳定表达的基因改造。标准的RNAi技术服务,包括设计与合成的siRNA的利益,验证siRNA的沉默效率,siRNA转染条件的优化,使高效的基因沉默细胞系或原代培养细胞的靶基因。转染培养细胞的瞬时或稳定的引入外源性分子和遗传物质(即RNA或DNA),通常是在生物实验室用来研究基因功能,基因表达的调节,生化映射,突变分析,和蛋白质的生产。科学家利用各种载体分子,这种分子,使质粒DNA(PDNA),信使RNA(mRNA),短干扰RNA(siRNA),小分子RNA(miRNA)的,并进入肿瘤细胞株和原代细胞的蛋白质的基因交付。不幸的是,无单提货的方法或转染试剂,可以适用于所有类型的细胞,细胞的细胞毒性和转染效率显着不同,取决于试剂,协议,并正在利用细胞类型。Altogen生物系统公司提供超过60种类型的细胞的预优化转染试剂盒。纳米粒子,脂质和聚合物基ALTOGEN®在体内转染试剂,使交付功能的RNA和DNA分子在体内。PEG脂质体在体内输送系统减少由于PEG修饰的先天免疫反应,并提供高效的siRNA转染的DNA,并在体内的蛋白质。由科学“杂志(2010年12月17日):PEG脂质体在体内转染试剂盒siRNA的特色Altogen生物系统功能的特定细胞系转染试剂盒
120+细胞转染试剂和活体组织靶向试剂盒制造商AltogenBiosystems是一家生物技术公司,开发和制造用于生命科学研究、药物发现和开发的转染试剂盒。Altogen®体内转染试剂可有效地将生物分子导入靶组织。细胞转染试剂盒针对特定的癌细胞系和原代细胞进行了优化。通过先进的试剂配方和优化的转染方案实现货物分子(DNA、RNA、蛋白质)的高效传递。AltogenBiosystems利用高分子化学、分子和细胞生物学的专业知识,开发了新的体内外给药技术。转染是将外源分子导入培养细胞中,常用于研究基因功能、基因表达调控、生化定位和蛋白质生产。不幸的是,由于细胞毒性和转染效率的差异很大,并且取决于所使用的试剂、方案和细胞类型,因此没有一种单一的传递方法或转染试剂可应用于所有类型的细胞。AltogenBiosystems为120多个癌细胞系和原代细胞类型提供优化的转染试剂盒和电穿孔产品。体内转染试剂可实现组织靶向给药。Altogen的转染试剂盒包括用于体外(癌细胞系)和体内(用于动物研究的组织靶向试剂)转染的转染增强剂试剂和转染复合物冷凝器。Altogen实验室提供符合GLP的实验室合同研究服务。我们的生物CRO服务包括异种移植物的疗效、IND应用的pharm/tox研究和安全性测试、分析开发(ELISA、IC-50、qPCR)、90多个异种移植物动物模型、RNAi和基因沉默服务。Altogen的细胞培养科学家通过在28天内培育出稳定的细胞系,将选择的细胞系转化为稳定表达感兴趣的基因。
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质粒转染一般是说DNA转染,用常用的sinofection等转染试剂就可以;
小干扰RNA(Small interfering RNA;siRNA)有时称为短干扰RNA(short interfering RNA)或沉默RNA(silencing RNA),是一个长20到25个核苷酸的双股RNA,这种RNA的转染需要用专门的RNA转染试剂。
DXY721认为:
悬浮细胞和贴壁细胞在转染过程中差别不大,主要差别在于转染后的筛选,当然如果你做的是瞬时转染就不存在筛选的问题了。
其实转染的过程很简单,问题是能不能转的进去的,转染率能有多少,转进去是否可以稳定表达目的蛋白等等。
我们也是用脂质体做悬浮细胞的转染,说明书上都有具体的操作过程,将脂质体和目的基因按比例混合,然后加到细胞悬液里就OK了,说的简单,实际上还是有一些细节要注意的,比如脂质体和目的基因混合的比例,转染的细胞数,细胞的代数,细胞的状态,有的还要求在转染的前一天传代一次,不过不要怕,这些在脂质体说明书上都有明确的说明,按照说明书做就可以了。
jinghuanlv认为:
悬浮细胞和贴壁细胞转染还是有很大不同的。
脂质体转染的原理基于电荷吸引原理,先形成脂质体-DNA复合物,散布在细胞周围,然后通过细胞的内吞作用,将目的基因导入细胞内,而脂质体复合物与贴壁细胞的接触机会比悬浮细胞高出很多倍,所以,脂质体转染时悬浮细胞的转染效率要明显低于贴壁细胞。
我们实验室转染悬浮细胞是用的电穿孔法,目前为止,悬浮细胞转染的最好方法还是电转,我们实验室用的电转仪是Bio-Rad的,使用条件是电压250V,电容975uF,效果不错,不妨一用。
近期,上海公卫临床研究中心的一位研究生应用两种转染试剂Turbofecttransfectionreagent(Thermo)和EntransterTM-R4000(Engreen)进行了一次RNA转染比较。比较情况如下:
实验方法
转染试剂:Turbofecttransfectionreagent(Thermo)和EntransterTM-R4000(EngreenBiosystemCo,Ltd)
待处理细胞:humanCD8+T细胞
1.针对Turbofect转染的方法
每孔培养体积均为100μL,细胞数目在105左右。
取0.5μLagomir,加入9.5μL无血清RPMI1640,充分混匀;
取0.2μLTurbofecttransfectionreagent和agomir稀释液充分混合。
转染复合物制备完成;混匀后室温下孵育15-20分钟,直接取10μL加入已铺好细胞的孔中,继续培养24h收取细胞,用PBS洗涤3次以上,以备RNA抽提。
转染Mix的配制:100nMago/N.C.:(0.5μLagomir+0.2μLTurbofect+9.3μL无血清的RPMI1640)×2.5=1.25+0.5+23.25
2.EntransterTM-R4000转染
每孔培养体积均为100μL,细胞数目在105左右。
取0.5μLagomir,加入9.5μL无血清RPMI1640,充分混匀,制成10μLagomir稀释液;
取0.25μLEntransterTM-R4000,然后加入9.75μL无血清稀释液体,充分混匀,制成10μLEntransterTM-R4000稀释液;
将EntransterTM-R4000稀释液和agomir稀释液充分混合(可用振荡器或加样器吹吸10次以上),室温静置15分钟。
转染复合物制备完成;
将20μL转染复合物加入孔中的细胞悬液中,前后移动培养皿,混合均匀;
转染后6h观察细胞状态,并更换培养基,继续培养24h后收取细胞,用PBS洗涤3次以上,以备RNA抽提。
转染Mix的配制:100nMago/N.C.:(0.5μLagomir+9.5μL无血清的RPMI1640)×2.5=1.25+23.75;Etranster稀释液:(0.25μLEtranster+9.75μL无血清的RPMI1640)×5=1.25+48.75(室温静置5分钟),取20μL至ago和N.C.管中,室温静置15分钟。
实验结果
结论:从目的miRNA表达水平的检测结果来看,转染24h后,英格恩生物公司(EngreenBiosystem)的EntransterTM-R4000(ago/NC:422912倍)转染试剂的效果优于Turbofect转染试剂(ago/NC:285870倍)。
讨论:从实验结果来看,英格恩生物公司(EngreenBiosystem)的EntranstenTM-R4000(ago/NC:422912倍)转染试剂的效果优于Turbofect转染试剂(ago/NC:285870倍)。EntransterTM-R4000是英格恩生物(EngreenBiosystem)最新研发合成的针对siRNA、microRNA、mimic、inhibitor、mRNA和shRNA等RNA的转染试剂。EntransterTM-R4000不仅可以转染小RNA,而且针对mRNA等长链RNA优化。该试剂可将RNA导入多种细胞系,包括原代细胞和悬浮细胞。无论有无血清、抗生素存在均可获得很高的转染效率。
而且直接使用自制的PEI非常便宜,在293上远比商品化的脂质体要好。
另外,如果你们实验室确实钱多,不怕花钱,建议你取用Promega的FugenHD,那个转染效率比脂质体更好,而且毒性小,至于价格。。。。。。。。。。。也更高。。。。。。。。。
另外,你说漂浮的细胞有表到GFP,那个不一定是真的GFP,很多时候
—DNA转染试剂Polyjet-适用于普遍的哺乳动物细胞
LipoD293-适用于悬浮细胞的转染(包括昆虫细胞SF9)-对于普遍的哺乳动物细胞具有更优异的转染效率
—DNAandsiRNA转染试剂
Lipojet-适用于大多数哺乳动物细胞的转染-低毒性(无需换液)-用量少-DNA/RNA共转染(co-transfection)-效果优于Lipofectamine2000
—siRNA转染试剂
GenMute-适用于大多数哺乳动物细胞转染-低毒性(无需换液)-用量少-DNA/siRNA共转染(co-transfection)PepMute-适用于普遍的哺乳动物细胞的转染