Cat.No.112-2P | 100 µg peptide, lyophilized. For reconstitution add 100µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should be stored at -20°C when still lyophilized! |
Immunogen | Synthetic peptide corresponding to AA 283 to 298 from mouse βSNAP (UniProt Id: P28663) |
Recommended dilution | Optimal concentrations should be determined by the end-user. |
Matching antibodies | 112203 |
Remarks | This control peptide consists of the synthetic peptide (IKKSIQGDGEGDGDLK) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
Data sheet | 112-2p.pdf |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
请教各位老师,
文献中检测细胞的活性有的用“NADPHdehydrogenase(NADPH脱氢酶)”有的用“NADPHdiaphorase(NADPH黄递酶)”
它们是同一种酶吗?它们的功能是什么?检测它们的活性是否能够评估细胞的活性。
不胜感激
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
明白了么,少年?
有谁用碧云天的过氧化氢酶检测试剂盒,在试剂盒的准备工作中,过氧化氢的实际浓度=22.94*A240,我测出来的吸光度是3.3左右,那么乘以22.94就等于76多点,再乘以之前的稀释倍数,大约就是7600mM左右,这样跟说明书中说道的1M相差太多,感觉不对啊,稀释的肯定是没有错的,但是不知道哪里出了错,怀疑说明书就有问题呢,有人做过这个实验吗?能不能准确测出过氧化氢浓度吗?有测过的人帮忙指点一下啊,不知道应该怎么做