- OutstandingperformanceandvalueareperfectforroutinePCR.
- Canbecycledupto98°CtoamplifythemostchallengingGC-richtemplatesotherscannot.
- Ready-to-usePCRreactionmastermixes,containdNTPsandPCREnhancer.
- Non-proofreADIngpolymerase
- EconoTaqPLUSGREENcontainstrackingdyesforgelelectrophoresis
- MoreeffectivethanotherTaqmastermixes.EconoTaqPLUSandPLUSGREEN2XMasterMixescontainaproprietaryPCRenhancer,sotheycansuccessfullyamplifytemplatesthatfailtoamplifywithotherPCRmastermixes(Figure1).HighdensityreactionbufferenablesdirectgelloadingfollowingPCR.
- ExcellentresultsinroutinePCR.EconoTaqPLUSGREEN&EconoTaqPLUScontainhighpurity,highactivityEconoTaqDNAPolymeraseforreliableamplificationoftemplatesupto5kb(Figure1).
- AmplifychallengingGC-richtemplates.These2XMasterMixescanbecycledupto98°CtoamplifychallengingGC-richtemplatesotherscannot(Figure2).
- ConvenienttrackingdyesinEconoTaqPLUSGREEN.Ona1%agarosegel,thebluedyemigratesatthesamerateasa5kbDNAfragment,andtheyellowdyemigratesat75bp.SeeFigure3.Ifdesired,thedyesareeasilyremovedbystandardDNApurificationproductsorethanolprecipitation.Forfluorescenceorabsorbancedetectionassays,EconoTaqPLUS2XMasterMixoffersthesamegreatperformancewithoutthedyes.
- FlexIBLe&easytouse.EconoTaqPLUSGREENandEconoTaqPLUSarepackagedin50reactionvialsthatcanbestoredintherefrigerator(+4ºC)forupto3months.Theyarestableforatleast10freeze-thawcycles.
- Lowprice!EconoTaqPLUSGREENisonly26¢per50µlPCRreaction…foreverything!EconoTaqPLUSGREEN&EconoTaqPLUSoffergreatvaluewhencomparedtoTaqmastermixes. EconoTaqPLUSGREEN&EconoTaqPLUSprices(see“OrderingandPricing”)arebasedona50µlPCR;usinga25µlreactionvolumewillstretchyourresearchbudgetevenfurther.
FreeSampleAvailable
EconoTaq®PLUSGREEN&EconoTaqPLUS2XMasterMixesofferPerformance,Convenience,ReliABIlity,andValueforRoutinePCR.
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Figure1.EconoTaqPLUSGREENMasterMixvs.GoTaq®Greenmastermix(Promega)inPCR.PCRwasperformedaccordingtothemanufacturer’srecommendationsusingprimersspecificfortheindicatedgenes.PanelA,0.7kbprokaryoticsingle-strandedDNAbindingprotein(genomicDNA);PanelB,2.7kbprokaryoticDNApolymeraseA(genomicDNA);PanelC,4.5kbgene(plasmidDNA). |
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Figure2.GC-richregionsfromhumangenomicDNAwerePCRamplifiedusing98°Cdenaturation. |
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Figure3.EasyvisualizationwithEconoTaqPLUSGREEN.Duringelectrophoresis,thegreenloadingcolorseparatesintoaslow-movingbluedyeandafast-movingyellowdye. |
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PleaseNote:
SomeapplicationsinwhichLucigen'sEconoTaqPLUSGREENandEconoTaqPLUS2XMasterMixescanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.
ORDERINFORMATION
UseEconoTaqPLUSGREEN2XMasterMixwhenthePCRproductswillbeanalyzedbyagarosegelelectrophoresisandethidiumbromidestaining.UseEconoTaqPLUS2XMasterMixwhenthePCRproductswillbeanalyzedbyabsorbanceorfluorescenceexcitation,toavoidpossibleinterferencebythedyes.EconoTaqPLUSGREEN&EconoTaqPLUSpricesarebasedona50µlPCRreaction.ebiomall.com
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半定量反转录-聚合酶链反应(semi-quantitative reverse transcription and polymerase Chain reaction ,SqRT-PCR)是近年来常用的一种简捷、特异的定量RNA测定方法,通过mRNA反转录成cDNA,再进行PCR扩增,并测定PCR产物的数量,可以推测样品中特异mRNA的相对数量。
定量RT-PCR(quantitative reverse transcription and polymerase Chain reaction ,qRT-PCR)是在用一步法或两步法,在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,最后通过标准曲线对未知模板进行定量分析的方法。
半定量RT-PCR需要跑电泳,根据条带亮度的强弱来判断模板拷贝数的高低或者是表达量的高低,而定量RT-PCR则无需电泳可以实时监测整个PCR的全程并且由给出的Ct值及Standard Curve来判断gene拷贝数的高低。
在另外的一组管子中,加入已知拷贝数的DNA同时扩增,每个管子中加入的数量是不同的,但是从最小数目到最大数目的这个范围涵盖了样本中DNA拷贝数。
PCR反应完成后,把已知拷贝数DNA的量和PCR荧光Ct值制成标准曲线,再把待测样本的CT值和该标准曲线比对,就可以得到样本中的起始拷贝数了
有大神做过人外周血全血miRNA的提取及后续PCR实验的吗?目前课题实验遇到瓶颈,求助大神!!!是否miRNA的PCR验证在全血做不出?必须要用血浆或者血清?目前血浆、血清也有部分样本。。。。有大神指条明路吗?



