| Product Name | SensoLyte ® AFC Plasmin Activity Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72124 |
| US$ | $380 |
Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis. The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity. Assays are performed in a convenient 96-well microplate format. Kit size: 100 assays | |
| Detailed Information | |
| Product Citations | Ambadapadi, S. et al. (2015) Reactive Center Loop (RCL) peptides derived from serpins display independent coagulation and immune modulating activities J. Biol. Chem. 291 2874-2887doi: 10.1074/jbc.M115.704841Portelli, MA. et al. (2014). Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels. FASEB J 28, 923.Park, HJ. et al. (2013). Identification of a specific haptoglobin C‐terminal fragment in arthritic synovial fluid and its effect on interleukin‐6 expression. Immunol 140, 133. doi: 10.1111/imm.12125.Sasaki, T. et al. (2012). Intravenous and oral administrations of DD2 [7-Amino-2-(sulfanylmethyl)heptanoic acid] produce thrombolysis through inhibition of plasma TAFIa in rats with tissue factor-induced microthrombosis. Thrombosis Res 130, e222. doi:10.1038/jid.2012.204Stewart, CE. et al. (2012). uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium. Thorax 67, 477.Tudpor, K. et al. (2012). Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria. J Am Soc Nephrol 23, 1824. |
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还需要反转录用的引物、定量PCR用的一对引物、荧光信号源(荧光染料或荧光标记的探针,看你用什么策略来做定量PCR)
报价要看你需要服务商提供什么服务,如果你只提供实验材料,要求服务商做以上全部工作,只检测一个指标(一个基因的表达水平变化),价格大约每个样品350-500元,多一个指标大约多90元。
一般至少做一组数据两个样品嘛,所以你按1000块一组样品预算吧。
PCR(聚合酶链式反应)是利用DNA在体外摄氏95°高温时变性会变成单链,低温(经常是60°C左右)时引物与单链按碱基互补配对的原则结合,再调温度至DNA聚合酶最适反应温度(72°C左右),DNA聚合酶沿着磷酸到五碳糖(5'-3')的方向合成互补链。
恒温PCR和实时荧光定量PCR的不同,是不同在实时荧光定量PCR的系统中加入了荧光染料(SYBR Green 或Taqman 探针等等)。以SYBR Green为例,这种染料可以结合在双链的DNA上,当PCR不断进行时,每一次退火生成的双链DNA也在增加,荧光染料结合也越多,荧光也越强。在机器中有一个探测荧光的探头,可以定量检测荧光的强度,转换成数值。这样就可以实时记录反映体系中DNA的反应情况。
荧光PCR更有优势,因为荧光PCR灵敏度高于恒温PCR,同样价格也高一些。
如果要测量细胞表面2个受体的比率,最好用流式细胞仪来测量
荧光定量PCR较普通PCR不同的一点就是可以实时检测PCR扩增产物,从而可进行绝对定量或者相对定量。
一般有ROX Reference Dye和ROX Reference Dye II两种,针对不同的PCR仪.一般ABI PRISM 7000/7700/7900HT和7300 Real-Time PCR System使用ROX Reference Dye,而7500 Real-Time PCR System和7500 Fast Real-Time PCR System使用ROX Reference Dye II.其它牌子如Thermal Cycler Dice Real Time System、LightCycler 等Real Time PCR扩增仪时不必使用.

