
Overview:
Leptin is a serum protein produced by adipocytes that play a main role in the regulation of body fat. Leptin is a growth factor in colonic epithelial cells and one that may provide a biological explanation for the observed associations between obesity, physical activity, and colon cancer (1). Leptin appears to be able to control the proliferation of both normal and malignant breast epithelial cells and used for normal mammary gland development (2).
Gene Aliases:
OB; OBS; FLJ94114; LEP
Genbank Number:
NP_000221
References:
1. Hardwick JC. et.al : Leptin is a growth factor for colonic epithelial cells. Gastroenterology. 2001 Jul;121(1):79-90.2. X Hu. et.al: Leptin--a growth factor in normal and malignant breast cells and for normal mammary gland development. J Natl Cancer Inst. 2002 Nov 20;94(22):1704-11.
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

