
Overview:
N6AMT2 is a putative DNA methyltransferase, belongs to the methyltransferase superfamily, AML1 family. The N6AMT2 gene is conserved in human, mouse, rat, chimpanzee, dog, cow, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, N.crassa, A.thaliana, and rice (1). The N6AMT2 phosphorylation at Ser-2 has been reported (2). The function of this protein has not been widely studied yet.
Gene Aliases:
ESP13
Genbank Number:
NM_174928
References:
1. Albrecht M, et.al: Novel Sm-like proteins with long C-terminal tails and associated methyltransferases. FEBS Lett. 2004 Jul 2;569(1-3):18-26. 2. Gauci,S. et.al: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal. Chem. 81 (11), 4493-4501 (2009).
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植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
但是,这样做的前提的,RNA提得要好,浓度要高,这样免得RNA降解到不能用了。因为在有二价阳离子的(DNAseI buffer含有)情况下,RNA在60度以上时必然发生非酶促降解。
想搞清一个问题,如何区别是因为small RNA 引起的调控呢?还是tRNA引起的调控,有没有有效的分离这两种RNA的办法呢?
样品处理:
a. 植物组织:取新鲜或-70℃冻存100mg组织在液氮中研磨,把粉末加入到1ml裂解液中混匀。
b. 动物组织:取新鲜或-70℃冻存100mg组织加1ml裂解液,用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞:直接在培养板中加入裂解液裂解细胞,每106细胞加1ml 裂解液。用取样器吹打混匀。

