
Overview:
SH2D1A contains an SH2 domain and a short tail, and expresses at a high level in thymus and lung. It acts as an inhibitor of the SLAM (signaling lymphocyte-activation molecule) proteins by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. It modifies signal transduction pathways in activated T, B and NK cells. SH2D1A mediates interaction between FYN and SLAMF1 (1). Mutations in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1) (2).
Gene Aliases:
DSHP; EBVS; IMD5; LYP; MTCP1; SAP; SAP/SH2D1A; XLP; XLPD
Genbank Number:
NM_001114937
References:
1. Chan B., et al: SAP couples Fyn to SLAM immune receptors. Nat. Cell Biol. 5:155-160(2003).2. Dutz, J. P., et al: Lymphocytic vasculitis in X-linked lymphoproliferative disease. Blood 97: 95-100, 2001.
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植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
但是,这样做的前提的,RNA提得要好,浓度要高,这样免得RNA降解到不能用了。因为在有二价阳离子的(DNAseI buffer含有)情况下,RNA在60度以上时必然发生非酶促降解。
想搞清一个问题,如何区别是因为small RNA 引起的调控呢?还是tRNA引起的调控,有没有有效的分离这两种RNA的办法呢?
样品处理:
a. 植物组织:取新鲜或-70℃冻存100mg组织在液氮中研磨,把粉末加入到1ml裂解液中混匀。
b. 动物组织:取新鲜或-70℃冻存100mg组织加1ml裂解液,用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞:直接在培养板中加入裂解液裂解细胞,每106细胞加1ml 裂解液。用取样器吹打混匀。

