
Technical Documents
Description
Glen-Pak™ DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5" terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. The Glen-Pak DNA 3mg 384-Well Plate is designed for use with 384-well plate compatible vacuum manifold systems and can purify up to a 20 nmole scale synthesis. Each well contains 3mg of Glen-Pak DNA resin, which binds about 15 nmoles of full length 40-mer DMT-ON oligo. Scale suggestions for the Glen-Pak DNA product line are shown below:
Glen-Pak DNA Product | Catalog Number | Synthesis Scale Compatibility | |
Glen-Pak DNA 50mg Purification Cartridge | 60-5000-96 | 0 nmole - 200 nmole | |
Glen-Pak DNA Purification Cartridge | 60-5100-XX and 60-5200-XX | 10 nmole - 1.0 µmole | |
Glen-Pak DNA Cartridge 3G | 60-5300-01 | 5 µmole - 20 µmole | |
Glen-Pak DNA 30 mg 96-Well Plate | 60-5400-01 | 10 nmole - 50 nmole | |
Glen-Pak DNA 3mg 384-Well Plate | 60-5500-xx | up to 20 nmoles |
A User Guide to Glen-Pak™ Purification describes in detail the process and several applications for DNA and RNA purification.This booklet is available online at: https://www.glenresearch.com/media/productattach/g/l/glen-pak_2.9_1.pdf.
Details
Specifications | |
---|---|
Storage | Controlled room temperature |
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
向左转|向右转

