
Lazy-L-SpreadersTM disposable microbiological spreaders provide an economical alternative to bending glass rods or pipets for spreading samples on agar surfaces and filters in Petri dishes. They are supplied sterile -- no need to flame sterilize or autoclave.unlike glass spreaders, durable polystyrene Lazy-L spreaders are non-breakable and pose no safety hazard L-shaped for convenient "lazy" spreading; just turn the Petri dish for smooth, even sample distribution smooth spreading surface and a slight upward turn reduce the chance that the spreader will dig into agar packaged in presterilized pouches of one or 10 each presterilization eliminates flaming or autoclaving and reduces risk of contamination no more time spent bending glass rods or waiting for glass or metal spreaders to cool before use!
ebiomall.com






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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。

