Hito CryoMyelinStain™ Plus Kit (Gold phosphate complex Myelin Staining Kit with Hematoxylin Counter Stain) is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons and nuclear counterstaining on frozen sections (mounted or floating). This kit has many advantages compared to the traditional Luxol fast blue staining method which is time-consuming, requires usage of 40-56°C incubator, and usually has low yields of stained myelin fibers and unreliable results. In addition, the long time high temperature incubation process of the frozen sections may cause sections felling off from the slides. Hito CryoMyelinStain™ Plus Kit offers a simple solution to these problems. The procedures are simplified and the processing time is greatly reduced. Users can use mounted sections or floating sections at room temperature. This kit delivers stable and improved staining quality. It has been proven to be extremely reliable and sensitive for demonstrating the morphological details of myelin fibers.
Myelin is essential for the proper functioning of the nervous system. Demyelination impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Hito CryoMyelinStain™ Plus Kit allows sensitive localization and visualization of the myelin fibers, thus offers a fast and reliable way to determine the extent of demyelination.
Hito CryoMyelinStain™ Plus Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
| Kit Contents (for 60 slides or 200 sections) | |
| Solution-1 | 50 ml |
| Solution-2 | 3 ml |
| Solution-3 | 12 ml |
| Solution-4 | 250 ml |
| Solution-5 | 25 ml |
| Solution-6 | 30 ml |
| Solution-7 | 125 ml |
| Staining Jar | 3 |
| Hito Aqua Barrier PAP Pen (HTHS0110) | 1 |
| Fine Tip Natural Hair Brush | 1 |
| Glass Specimen Transfer Tool | 1 |
| User Manual and MSDS | 1 |
Before using Hito CryoMyelinStain Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat and light microscopeDry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized waterGelatin coated slides and coverslipsStaining jars for slides washResinous mounting medium  Hito CryoMyelinStain™ Plus Kit Manual and MSDS | |
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我在一些文献上看到说，要加入tRNA as carrier，但是这句话不好理解
有没有人用过，跟我讲讲tRNA到底有什么用
多谢指教
人教版高中生物必修二有这个图
想搞清一个问题，如何区别是因为small RNA 引起的调控呢？还是tRNA引起的调控，有没有有效的分离这两种RNA的办法呢？
样品处理：
a. 植物组织：取新鲜或-70℃冻存100mg组织在液氮中研磨，把粉末加入到1ml裂解液中混匀。
b. 动物组织：取新鲜或-70℃冻存100mg组织加1ml裂解液，用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞：直接在培养板中加入裂解液裂解细胞，每106细胞加1ml 裂解液。用取样器吹打混匀。
你可以用你的目的引物,用反转录第一链进行扩增,看看能不能得到目的片段.
1、测浓度和纯度，是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足，就不需要纯化，否则需要进一步纯化。
不过根据经验，一般都不需要，我们实验室用的是GNT系列的试剂盒，提取结果就直接进入下游实验了，效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器（主要指线粒体和叶绿体）中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌）、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
1、测浓度和纯度，是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足，就不需要纯化，否则需要进一步纯化。
不过根据经验，一般都不需要，我们实验室用的是GNT系列的试剂盒，提取结果就直接进入下游实验了，效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器（主要指线粒体和叶绿体）中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌）、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
实验非常不顺，想构建CDNA文库，但是从mRNA开始屡次失败，考虑主要是纯化过程中损失过多。想从总RNA入手，但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶，苦于没有第二连合成试剂盒，不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时，不需要另外的引物，利用降解的RNA作引物即可，我想直接设定PCR两个循环，合成第二链，不知方法可行否？愁啊，等着毕业，时间紧急，恳请帮忙。谢谢。
