DescriptionViPrimePLUS One Step Taq RT-qPCR Master Mix is next generation master mix designed for one step realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step Taq RT-qPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.ViPrimePLUS One Step Taq RT-qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RTqPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Master Mix is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.
Features
- One step real time RT-qPCR reaction set up
- Equipped with thermostable M-MULV enzyme
- Good buffer system for excellent amplification efficiency
- High sensitivity detection
- Optimal performance for highly sensitive and specific RT-qPCR reaction
- Compatible with most of the real-time PCR platforms
Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step Taq RTqPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM13ViPrimePLUS One Step Taq RT-qPCR Master Mix | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM13-LRViPrimePLUS One Step Taq RT-qPCR Master Mix with Low ROX | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM13-RViPrimePLUS One Step Taq RT-qPCR Master Mix with ROX | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM13 | ViPrimePLUS One Step Taq RT-qPCR MasterMix | 150 reactions |
| QLMM13-LR | ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX | 150 reactions |
| QLMM13-R | ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX | 150 reactions |
DownloadManual
ViPrimePLUS One Step Taq RT-qPCR MasterMix ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX
PublicationThis Product Has Been Used In:Attaran, B., Falsafi, T. (2017).). Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics, . World Journal of Gastroenterology, Vol. 23, No. 7. 1163-1170 (2017).
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植物材料 用最精确的方法，称取不超过100mg的植物材料，置于处理过的研钵，加入液氮进行研磨 将研磨得到的粉末，快速转移至无RNase，并经过液氮冷却的2mL离心管(自备)，
但是，这样做的前提的，RNA提得要好，浓度要高，这样免得RNA降解到不能用了。因为在有二价阳离子的（DNAseI buffer含有）情况下，RNA在60度以上时必然发生非酶促降解。
想搞清一个问题，如何区别是因为small RNA 引起的调控呢？还是tRNA引起的调控，有没有有效的分离这两种RNA的办法呢？
样品处理：
a. 植物组织：取新鲜或-70℃冻存100mg组织在液氮中研磨，把粉末加入到1ml裂解液中混匀。
b. 动物组织：取新鲜或-70℃冻存100mg组织加1ml裂解液，用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞：直接在培养板中加入裂解液裂解细胞，每106细胞加1ml 裂解液。用取样器吹打混匀。
