Benchmark Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp). It's low EEO (= 0.13-mr ) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions. Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio. Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added. Gel Strength: >1200g/cm2 (1%)Gel Temperature: 360C + 1.50C (1.5%)Melt Temperature: 8800C + 1.50C (1.5%)EEO(-mr): 0.13Moisture Content: <7%Sulfate: <0.2%RNase/DNase: None DetectedProtease/Endonuclease: None DetectedStorage Conditions: Room Temperature
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比如，你的流动相比例、水相pH值、柱温等等条件稍有变化，色谱峰就对不上了。比如，一个碱性物质检测，流动相的pH值稍有波动，这个碱性物质的保留时间就前后波动的很厉害。这个时候就需要定位。
标准品：一般来说，标准品的结构是确定的，含量是确定的。结构就不用说了，保留时间一致就代表是这个东西了。含量，用外标的话就可以计算出样品的含量了。
这个定位品……我倒是没接触过定位品，就理解成用来定位的物质吧。标准品价格比较贵，自制的标准品通过结构确证可以用来定位。这样确保这个位置的色谱峰是这个物质。
我做实验的药物很难买到标准品，但现在有粗品，自己在提纯，想问下各位大牛，在没有标准品的情况下，怎么测提纯后该物质的纯度。谢谢
