
Estradiol Antibody
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Product Description | This antibody was developed using β-estradiol-6-one-6-(O-carboxymethyloxime)-KLH conjugates as the immunogen and affinity purified with antigen-specific affinity chromatography. The antibody could be utilized for detection, quantization and affinity isolation and purification of estradiol in biofluid. | ![]() Structure of the Antigen | |
Species | Rabbit | ||
Formulation | 1000 or 250 µg/mL in PBS with 50% glycerol | ||
Immunogen | β-estradiol-6-one6-(O-carboxymethyloxime)-KLH conjugates | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes free estradiol. | ||
Applications | Quantitative ELISA,Immunoaffinity Chromatography | ||
Scientific Description | Estradiol (17β-estradiol) (also oestradiol) is a sex hormone which represents the major estrogen in humans. Estradiol not only has a critical impact on reproductive and sexual functions, but also affects other organs including bone structure. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored. |
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比如,你的流动相比例、水相pH值、柱温等等条件稍有变化,色谱峰就对不上了。比如,一个碱性物质检测,流动相的pH值稍有波动,这个碱性物质的保留时间就前后波动的很厉害。这个时候就需要定位。
标准品:一般来说,标准品的结构是确定的,含量是确定的。结构就不用说了,保留时间一致就代表是这个东西了。含量,用外标的话就可以计算出样品的含量了。
这个定位品……我倒是没接触过定位品,就理解成用来定位的物质吧。标准品价格比较贵,自制的标准品通过结构确证可以用来定位。这样确保这个位置的色谱峰是这个物质。
校准品:公司指定用校准某检测系统(仪器+试剂+程序)考虑具基质效应情况赋予校准品校准值校准品必须专用于某检测系统北京标准物质网:www.biaowu.com