Pig Universal Reference cDNA-Oligo dT is a full length first strand cDNA synthesized from Zyagen universal reference total RNA which is isolated from wide variety of male and female major tissues including brain, heart, kidney, liver, lung, skeletal muscles, pancreas, skin, stomach, intestine, colon, testis, ovary and uterus.
The cDNA is primed with Oligo dT primer and can be used in gene cloning, target sequencing and as a control for gene expression analysis by regular PCR and real time PCR. Each cDNA product is enough for 40 PCR reactions.
Features:
- Synthesized from high quality intact universal reference total RNA
- Consistent control for standard data set comparisons
- Prepared from several major tissues for broad gene coverage
- Made at industrial scales to minimize lot-to-lot variation.
Quality Control: The integrity of each RNA sample used for cDNA synthesis is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also used as template PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.
Applications: cDNA is ideal as a control for gene expression analysis by real time and regular PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.
Packing/shipping: each universal reference cDNA sample is routinely shipped on dry ice in 1.5 ml vials and is enough for 40 reactions (40 PCR amplifications).
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
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药品标准物质由中国药品生物制品检定所负责标定。中国药品生物制品检定所可以组织有关的省、自治区、直辖市药品检验所、药品研究机构或者药品生产企业协作标定国家药品标准物质。
药品标准物质分类包括：标准品、对照品、对照试剂、对照制剂、对照药材、标准量具或耗材、工作标准品、精品。
如果你需要购买 药品对照品、标准品，可以到中国药品生物制品检定所订购，也可以查询中国标准物质网订购。
比如，你的流动相比例、水相pH值、柱温等等条件稍有变化，色谱峰就对不上了。比如，一个碱性物质检测，流动相的pH值稍有波动，这个碱性物质的保留时间就前后波动的很厉害。这个时候就需要定位。
标准品：一般来说，标准品的结构是确定的，含量是确定的。结构就不用说了，保留时间一致就代表是这个东西了。含量，用外标的话就可以计算出样品的含量了。
这个定位品……我倒是没接触过定位品，就理解成用来定位的物质吧。标准品价格比较贵，自制的标准品通过结构确证可以用来定位。这样确保这个位置的色谱峰是这个物质。
