Sheep UniversalReference CDNA-OligodT isa fulllengthfirststrandcDNAsynthesizedfromZyagenuniversalreferencetotalRNAwhichis isolatedfromwidevarietyofmaleandfemalemajortissuesincludingbrain,heart,kidney,liver,lung,skeletalmuscles,pancreas,skin,stomach,intestine,colon,testis,ovaryanduterus.
ThecDNAisprimedwithOligodTprimerandcanbeusedingenecloning,targetsequencingandasacontrolforgeneexpressionanalysisbyregularPCRandrealtimePCR. EachcDNAproductisenoughfor40PCRreactions.
Features:
- SynthesizedfromhighqualityintactuniversalreferencetotalRNA
- Consistentcontrolforstandarddatasetcomparisons
- Preparedfromseveralmajortissuesforbroadgenecoverage
- Madeat industrialscalesto minimizelot-to-lotvariation.
QualityControl: TheintegrityofeachRNAsampleusedforcDNAsynthesisisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.
ThesynthesizedcDNAisalsousedastemplatePCRamplificationofß-actingene.PCRproductofß-actinwasvisualizedon1%agarosegel.
Applications: cDNAisidealasacontrolforgeneexpressionanalysisbyrealtimeandregularPCR,characterizationofalternativesplicingofmRNA,verificationofgeneticmutation,genecloningandtargetsequencing.
Packing/shipping:eachuniversalreferencecDNAsampleisroutinelyshippedondryicein1.5mlvialsandisenoughfor40reactions(40PCRamplifications).
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com
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