
Zyagen MonkeyUniversalReferenceRNAiscomposedofhighlypureintacttotalRNAisolatedfrommajormaleandfemale Cynomolgusmonkeytissuesincludingbrain,heart,kidney,liver,lung,skeletalmuscles,pancreas,spleen,stomach,intestine,colon,testis,ovaryanduterus.TotalRNAisextractedusingmodifiedguanidinethiocyanatetechniquewhichallowstherapidisolationoftotalRNAincludingmicroRNAs.Tissuesarecutinverysmallpieces,snapfrozen,grindingtopowder,andhomogenizedinRNAdenaturedsolutionforRNApurification.TheextractedRNAistreatedwithDNase,packedataconcentrationof1mg/ml,andstoredat-80oC
Features:
- HighqualityintactRNAwithoutgenomicDNAcontamination
- Consistentcontrolforstandarddatasetcomparisons
- Extractedfromlargevarietyofmaleandfemale monkeymajortissuesforbroadgenecoverage
- Madeat industrialscalesto minimizelot-to-lotvariation
QualityControl: TheintegrityofRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.
Applications: Reference RNAisidealformicroarrayanalysis,Northernblotting,ribonucleaseprotectionassay,SInucleaseassay,RT-PCR/Q-PCR/RACEanalysis,CDNAsynthesis,RNAdifferentialdisplay,microRNAstudies,andpurificationofmRNAforlibraryconstruction.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof1mg/mlandshippedondryice.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com
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Reference material, accompanied by a certificate, one or more of whose property values are certified by a procedure, which established its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence. (Brammer StandardBASARMIetc). (ISO Guide 30-1992)
标准品CRM:标准物质/标准品附带析证书属性值某项或若干项都按照某程序进行验证验证程序建立些属性值所表述体系精确体现追溯性每经验证数值都伴随着某特定置信水平确定度(BS,NISTBASARMI等)(ISO Guide 30-1992)
Reference Materials(RM)
Materials or substance one or more of whose property values are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning values to materials. (ISO Guide 30-1992)
照品RM:种原料或物质其某种或几种属性值表现充均性并且用于设备校准析评估或者物质定植(ISO Guide 30-1992)
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