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Exocell/m Albumin/1011 Strip Plate
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4000-520-616
mAlbumin | |
Manufacturer/Trade: | Exocell/AlbuwellM |
CatalogueNumbers: | 1011StripPlate |
Methodology: | CompetitiveELISA |
Summaryofprocedure: | AlbuwellMisadirectcompetitiveELISA(antibodycapture)designedtomonitorkidneyfunctioninthemousebymeasuringurinaryalbumin.Tocompletetheassay,sampleandanti-mousealbuminAb-HRPconjugateareaddedtoalbumincoatedwells.Theantibodyconjugatereactswitheitherthealbumincoatedtotheplateorwiththatinthefluidphase,hencethenotionofcompetitivebinding. Aftera30minuteincubation,theplateiswashedtoremovereactantsinthefluidphase.Onlyconjugatethatwasboundtothealbuminontheplateremains,anditisdetectedusingTMBinachromogenicreaction.Colorintensityisinverselyproportionaltothelogarithmofthealbuminconcentrationinthefluidphase.Theassaymaybecompletedinlessthananhour. |
SpecimenRequired: | Urine,10ul |
AssayRange: | 0.156-10ug/mL |
Precision: | IntrassayandinterassayprecisionforsampleswithinusefulrangeoftheassayhaveaC.V.≤10%ofthemean. |
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