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Exocell/DTNB-Thiols/1021
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4000-520-616
DTNB-Thiols | |
Manufacturer/Trade: | Exocell/DTNB-Thiols |
CatalogueNumbers: | 1021 |
Methodology: | ChemicalAssay |
Summaryofprocedure: | Thethiolsassaykitmeasuressulfhydrylgroupswiththethiolreagent5-5"-dithiobis[2-nitrobenzoicacid](DTNB),whichformsthe5-thionitrobenzoicacidandamixeddisulfide.Underconditionsofoxidativestress,freesulfhydryldecreasesanddisulfidesincrease.DeterminationofthefreethiolconcentrationinBIOLOGicalsamplesreflectstheABIlitytodetoxifylipidandotherperoxides.Exocell"sDTNB-Thiolsassaycanbeusedwithaspectrumofbiologicalsamplesincludingbodyfluids,tissueandcellspecimens. Tocompletetheassay,aliquotsofstandardorsamplearereactedwithrehydratedDTNBreagentinmicrotiterwells.Absorbanceisreadat405nmusingareferencefilterof~630nm.Concentrationinsamplesisdeterminedfromthestandardcurve. |
SpecimenRequired: | 50uL |
AssayRange: | 0-56.9nmole/well |
Precision: | Intra-andinter-assayprecision<5% |
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