The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) createdin a cell population using nuclease-based genome editing technologies, including CRISPR/Cas9, TALENs, and zinc finger nucleases. The kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. The indel identification protocol in this kit has been optimized to provide quick and reliable results.
The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) createdin a cell population using nuclease-based genome editing technologies, including CRISPR/Cas9, TALENs, and zinc finger nucleases. The kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. The indel identification protocol in this kit has been optimized to provide quick and reliable results. First, Terra PCR Direct polymerase is used to amplify a genomic DNA fragment containing the target site directly from crude cell lysates. The resulting amplified fragments are cloned directly, without restriction digestion, into a pre-linearized pUC19 vector using the In-Fusion Cloning system. After transformation into Stellar Competent Cells, colony PCR is used to amplify the target region from the plasmid. DNA sequence analysis of the PCR products is then used to identify indels generated at the targeted genomic site.
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点：我看了Elisa的说明书，现在检测PDGF有两种试剂盒，一种是检测PDGF的，要求的试剂样本为组织匀浆；一种是检测PDGF-BB的，要求的待测样本为血液或血浆。
我现在的实验目的是想更精确，检验组织中的PDGF-BB，但是手中的是人体韧带组织样本，非PDGF-BB试剂盒所要求的组织样本为血浆或血清，我不知道是否可以用PDGF-BB的ELISA试剂盒的来做，所以特地请教：）
请教第二点：市面上有RB公司的96T，2500元；另外有Uscnlife的Elisa试剂盒，96T的3680元，都说是原装进口的，我该如何选择呢？为何差价这么多？谢谢指点！
一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
