L-Arginine ELISA Assay Kit
L-Arginine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol
Size: 1×96 wells
Sensitivity: 2.1 µM
Standard Range: 5.12-200 µM
Incubation Time: Overnight
Sample Type: Serum, Plasma and Cell Culture
Sample Size: 20µL
Alternative Names: Arginine ELISA, L-Arg,
For Research Use Only
Controls Included
Background
Enzyme Immunoassay for the quantitative determination of L-Arginine (Arg) in plasma samples. After extraction and derivatization Arginine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
Specificity: No significant cross-reactivity was observed with L-Homoarginine, Agmatine, L-Citrulline, L-Ornithine, ADMA or SDMA
Assay Principle
The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
Products Related to L-Arginine ELISA Assay Kit
ADMA Arginine ELISA Assay Kit
Homoarginine ELISA Assay Kit
ADMA ELISA Assay Kit
SDMA ELISA Assay Kit
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点：我看了Elisa的说明书，现在检测PDGF有两种试剂盒，一种是检测PDGF的，要求的试剂样本为组织匀浆；一种是检测PDGF-BB的，要求的待测样本为血液或血浆。
我现在的实验目的是想更精确，检验组织中的PDGF-BB，但是手中的是人体韧带组织样本，非PDGF-BB试剂盒所要求的组织样本为血浆或血清，我不知道是否可以用PDGF-BB的ELISA试剂盒的来做，所以特地请教：）
请教第二点：市面上有RB公司的96T，2500元；另外有Uscnlife的Elisa试剂盒，96T的3680元，都说是原装进口的，我该如何选择呢？为何差价这么多？谢谢指点！
一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
