
Optimal Concentration | 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions |
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Application | PCR |
The performance of Betaine Enhancer Solution is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents because of the following effects: Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level.
At the same time, Betaine has a sequence independent destabilizing effect on all DNA. The Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered.
Furthermore, Betaine aids the process of thermostable polymerases and reduces pauses in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.
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一、免疫组化试剂盒一般包括:
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别,所以购买时一定了解清楚:
1、一抗是做人的还是兔、鼠的组织;
2、检测系统是ABC/SP/非生物素/其他;
3、试剂盒的内容都有什么?有没有显色系统?
4、如果没有,自己根据前面的检测系统需配哪个显色系统。(其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配)
三、免疫组化抗体即指特异性一抗,是用来标记的指标,免疫组化过程的其他试剂一律另外配备。
另外:做实验时,如遇出结果困难,记得做阳性片对照,敢于怀疑检测系统或显色系统的问题。
用双氧水去除过氧化物酶时,我是自己配的3%双氧水滴到片上而非将片浸入双氧水,这样可否?
用博士德的DAB染色,配制方法为:1mlH20中加入DAB、H2O2、TBS各一滴,我配制的时候发现将DAB加入H20时很容易沉淀,要震荡才能混匀,且将配好的DAB滴到玻片上时,DAB会结为很小块的微颗粒悬浮在液体中但并不染色,是否DAB有问题?
现在打算换中衫的试剂盒,代理商说中衫的试剂盒包括了二抗和DAB,想请教一下该试剂盒的具体内容有些什么?
万分感谢!
上海好多的供应商的,具体还是看你选择了,其实试剂盒产品都差不多,什么远慕生物,古朵生物...好多的额

