- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody against DYKDDDDK tag
Product Type:Purified primary antibodies
Target Protein:DYKDDDDK (Flag) tagged recombinant protein
Immunogen:A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Myeloma:Sp2/0-Ag14
Specificity:This monoclonal antibody binds to DYKDDDDK-tagged recombinant protein.
Cross-Reacitvity:Not identified
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2 with 0.05% sodium azide
Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Aliquot and store at -20oC for long term storage. Avoid repeated freeze and thaw cycles.
Research Area:Life science
Application:
Western blotting: 1:1,000.
Western blot analysis of 293 cells transfected with DYKDDDDK- tagged vectors at different concentration, Lane 4: Lowest concentration (0.1 μg/mL).
Immunoprecipitation and Immunostaining: 1:200.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-DYKDDDDK (Flag) Tag, Flag.C2 |
|---|---|
| Application | WB, IHC |
| Size | 0.1 mg |
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一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
