- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody against DYKDDDDK tag
Product Type:Purified primary antibodies
Target Protein:DYKDDDDK (Flag) tagged recombinant protein
Immunogen:A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Myeloma:Sp2/0-Ag14
Specificity:This monoclonal antibody binds to DYKDDDDK-tagged recombinant protein.
Cross-Reacitvity:Not identified
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2 with 0.05% sodium azide
Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Aliquot and store at -20oC for long term storage. Avoid repeated freeze and thaw cycles.
Research Area:Life science
Application:
Western blotting: 1:1,000.

Western blot analysis of 293 cells transfected with DYKDDDDK- tagged vectors at different concentration, Lane 4: Lowest concentration (0.1 μg/mL).
Immunoprecipitation and Immunostaining: 1:200.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-DYKDDDDK (Flag) Tag, Flag.C2 |
|---|---|
| Application | WB, IHC |
| Size | 0.1 mg |
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点:我看了Elisa的说明书,现在检测PDGF有两种试剂盒,一种是检测PDGF的,要求的试剂样本为组织匀浆;一种是检测PDGF-BB的,要求的待测样本为血液或血浆。
我现在的实验目的是想更精确,检验组织中的PDGF-BB,但是手中的是人体韧带组织样本,非PDGF-BB试剂盒所要求的组织样本为血浆或血清,我不知道是否可以用PDGF-BB的ELISA试剂盒的来做,所以特地请教:)
请教第二点:市面上有RB公司的96T,2500元;另外有Uscnlife的Elisa试剂盒,96T的3680元,都说是原装进口的,我该如何选择呢?为何差价这么多?谢谢指点!

