| Product Name | 5(6) - TAMRA, SE5 - (and - 6) - Carboxytetramethylrhodamine, succinimidyl ester; 5(6) - TAMRA, NHS ester |
| Size | 25 mg |
| Catalog # | AS-81124 |
| US$ | $134 |
Tetramethylrhodamine (TMR) is one of the fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. TMR dyes have been widely used as acceptors for FAM fluorophores in a variety of FRET studies. The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents.
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| Detailed Information | |
| Storage | -20°C desiccated and protected from light |
| References | 1. Hahn M, et al. Electrophoresis 22, 2691-700;(2001).2. Hsu TM, et al. Clin Chem 47, 1373-7; (2001). |
| Molecular Weight | 527.53 |
| Molecular Formula | C29H25N3O7 |
| Spectral Properties | Abs/Em = 546/575 nm |
| Solvent System | DMF or DMSO |
| Product Citations | Christie, R. et al. (2009). Optical Properties and Application of a Reactive and Bioreducible Thiol-Containing Tetramethylrhodamine Dimer. Bioconjugate Chem 20, 476. |
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点:我看了Elisa的说明书,现在检测PDGF有两种试剂盒,一种是检测PDGF的,要求的试剂样本为组织匀浆;一种是检测PDGF-BB的,要求的待测样本为血液或血浆。
我现在的实验目的是想更精确,检验组织中的PDGF-BB,但是手中的是人体韧带组织样本,非PDGF-BB试剂盒所要求的组织样本为血浆或血清,我不知道是否可以用PDGF-BB的ELISA试剂盒的来做,所以特地请教:)
请教第二点:市面上有RB公司的96T,2500元;另外有Uscnlife的Elisa试剂盒,96T的3680元,都说是原装进口的,我该如何选择呢?为何差价这么多?谢谢指点!


