The body’s immune response to foreign material helps to protect the body from disease and injury through the innate and adaptive immune systems. The innate immune system non-specifically protects against foreign particles through the use of protective barriers, inflammation, and the complement system. Cytokines and chemokines are produced in the innate immune response to promote inflammation and recruitment of T cells to trigger the adaptive immune response. The adaptive immune system protects against specific pathogens leading to the production of memory B-cells and T-cells. The cytokines produced in the adaptive immune response help to promote the survival of B-cells and T-cells and activate effector T-cells. Accurate measurement of these cytokines is essential for understanding immune responses and disease processes.The LEGENDplex™ Human Essential Immune Response Panel (13-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 13 key targets essential for immune response such as IL-4, IL-2, CXCL10 (IP-10), IL-1β, TNF-α, CCL2 (MCP-1), IL-17A, IL-6, IL-10, IFN-γ, IL-12p70, CXCL8 (IL-8), and Free Active TGF-β1. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant and serum.The LEGENDplex™ Human Essential Immune Response Panel (13-plex) is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.This assay is for research use only.
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点：我看了Elisa的说明书，现在检测PDGF有两种试剂盒，一种是检测PDGF的，要求的试剂样本为组织匀浆；一种是检测PDGF-BB的，要求的待测样本为血液或血浆。
我现在的实验目的是想更精确，检验组织中的PDGF-BB，但是手中的是人体韧带组织样本，非PDGF-BB试剂盒所要求的组织样本为血浆或血清，我不知道是否可以用PDGF-BB的ELISA试剂盒的来做，所以特地请教：）
请教第二点：市面上有RB公司的96T，2500元；另外有Uscnlife的Elisa试剂盒，96T的3680元，都说是原装进口的，我该如何选择呢？为何差价这么多？谢谢指点！
一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
用双氧水去除过氧化物酶时，我是自己配的3%双氧水滴到片上而非将片浸入双氧水，这样可否？
用博士德的DAB染色，配制方法为：1mlH20中加入DAB、H2O2、TBS各一滴，我配制的时候发现将DAB加入H20时很容易沉淀，要震荡才能混匀，且将配好的DAB滴到玻片上时，DAB会结为很小块的微颗粒悬浮在液体中但并不染色，是否DAB有问题？
现在打算换中衫的试剂盒，代理商说中衫的试剂盒包括了二抗和DAB，想请教一下该试剂盒的具体内容有些什么？
万分感谢！
