
Salmonellais an important intestinal bacterial pathogen and is widely distributed in nature. There are over 2,500 different serotypes of Salmonella.In recent years, the incidence of food poisoning caused by Salmonella (an illness referred to as Enteritis salmonellosis) has increased dramatically.The TaKaRa qPCR Salmonella Detection Kit China Standard is based on the industry standard of Entry-Exit Inspection and Quarantine of the People’s Republic of China (method: “SN/T 1059.7-2010 real-time fluorescence PCR method developed for the qualitative detection of Salmonella by Real Time PCR”). This qPCR kit is formulated as a premix at a 2X concentration, which simplifies PCR reaction assembly. The premix includes Takara Ex Taq HS, a hot-start enzyme and buffer system that effectively suppresses non-specific PCR amplification, greatly improving PCR amplification specificity and efficiency. The TaKaRa qPCR Salmonella Detection Kit China Standard offers high sensitivity for simple, fast, and specific detection of Salmonella by real-time PCR.
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一、免疫组化试剂盒一般包括:
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别,所以购买时一定了解清楚:
1、一抗是做人的还是兔、鼠的组织;
2、检测系统是ABC/SP/非生物素/其他;
3、试剂盒的内容都有什么?有没有显色系统?
4、如果没有,自己根据前面的检测系统需配哪个显色系统。(其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配)
三、免疫组化抗体即指特异性一抗,是用来标记的指标,免疫组化过程的其他试剂一律另外配备。
另外:做实验时,如遇出结果困难,记得做阳性片对照,敢于怀疑检测系统或显色系统的问题。

