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Description
Details
Description:Mouse monoclonal antibody to human immunoglobulin (Ig) Kappa (κ) light chain
Purification: Prootein G affinity purified
Product Type:Primary antibody
Target Protein:Free Kappa chain and combined Kappa light chain of human immunoglobulin
Immunogen:Human immunoglobulin
Fusion Myeloma:Sp2/0-Ag14
Specificity:Reactive with human Kappa light chain of human immunoglobulin in immunoblot and ELISA.
Reactivity: Human, others not tested
Host / Isotype:Mouse, IgG1 Kappa
Formulation:Lyophilized product
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.
Storage: Store at -20oC
Research Area:Immunology
Background:
The basic unit of human immunoglobulin (also called antibody) is comprised of 2 identical heavy chains and 2 identical light chains linked together by disulfide bonds. There are five types of heavy chains designated as α, δ, ε, γ, and μ, which define the class of the antibodies respectively as IgA, IgD, IgE, IgG and IgM. There are two types of light chain, designated as: k (kappa) and (λ) lambda which are defined by the constant region on light chain. Human kappa light chain genes are mapped to chromosome 2 and lambda light chain genes are mapped to chromosome 22.
Applications: ELISA,Immuno-blot
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human Ig Kappa Light Chain, YCK1 |
|---|---|
| Application | EIA, Immuno-Blot |
| Size | 0.1 mg |
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本人对软骨石蜡切片做了MMP-13的免疫组化染色，首次做，无法判断染色部位，请各位帮忙看一下
最近开始做TUNEL染色，第一次按罗氏protocol做的，阴性对照是没有显色的。没做阳性对照。第二次、第三次做出来背景都很深，肾小管每个细胞核上都染上了棕黄色，DAB染色很快，几秒就变一片黄。具体步骤是：1烤片脱蜡至水2.蛋白酶K20ug/ML37℃10分钟3.tunel液冰上配置后加组织上，37度1h。4.pod液37℃30min5.DAB显色。在蚂蚁淘中检索后，优化了实验条件，在蛋白酶K前加了3%H2O2封闭10min，缩短了tunel液至40min。这是第三次的结果大家帮我看看
这是对照组
这是模型组
