- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody against V5 tag, an epitope of Simian virus 5 RNA polymerase a.
Product Type:Purified primary antibodies
Target Protein:V5 tagged recombinant protein
Immunogen:A synthetic peptide representing amino acid residues 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of Simian virus 5 (SV5), coupled to KLH.
Fusion Myeloma:Sp2/0-Ag14
Specificity:This monoclonal antibody binds to V5-tagged recombinant protein.
Cross-Reacitvity:Not identified
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in pH 7.2 0.01M PBS with 0.05% sodium azide.
Storage:Aliquot and store at -20 oC for long term storage. Avoid repeated freeze and thaw cycles.
Research Area:Life science
Application:
Western blotting: 1:1,000 - 10,000.

Western blot analysis of 293 cells transfected with a lacZ-V5-tagged vector (2) and untransfected control (1).
Immunochemistry: 1:100 - 1:400
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-V5 Tag, V531 |
|---|---|
| Application | WB, IHC |
| Size | 0.1 mg |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
本人对软骨石蜡切片做了MMP-13的免疫组化染色,首次做,无法判断染色部位,请各位帮忙看一下
最近开始做TUNEL染色,第一次按罗氏protocol做的,阴性对照是没有显色的。没做阳性对照。第二次、第三次做出来背景都很深,肾小管每个细胞核上都染上了棕黄色,DAB染色很快,几秒就变一片黄。具体步骤是:1烤片脱蜡至水2.蛋白酶K20ug/ML37℃10分钟3.tunel液冰上配置后加组织上,37度1h。4.pod液37℃30min5.DAB显色。在蚂蚁淘中检索后,优化了实验条件,在蛋白酶K前加了3%H2O2封闭10min,缩短了tunel液至40min。这是第三次的结果大家帮我看看
这是对照组
这是模型组

